What should I consider when selecting a method of mitochondrial isolation?

Factors one should consider when selecting a method of mitochondrial isolation include function, downstream analysis, efficiency,  purity, yield, and the characteristics of the sample. 

1. The chosen method should preserve the overall function of mitochondria. This involves maintaining their ability to produce ATP and carry out respiratory processes. 
2. Efficiency refers to how effectively the method isolates mitochondria from other cellular components. An efficient method minimizes contamination and ensures a high yield of mitochondria. 
3. Different downstream analyses may require mitochondria with specific characteristics. 
4. Purity refers to the degree to which isolated mitochondria are free from contamination. A high-purity isolation method is crucial for accurate and reliable experimental results. 
5. Yield is the quantity of mitochondria obtained through the isolation process. 
6. The nature of the starting material (e.g. tissue) influences the choice of isolation method. Some methods may be better suited for specific sample types, and one should consider type, size, and cell density.