What should I do to optimize my cell culture media?

There are several steps involved in optimizing cell culture media. 

1. Use empirical or statistical methods to screen various base media to determine which ones best support the cell growth and productivity. 
2. Vary the concentration of individual components in the base media and observe the effect on cell growth and productivity. This will enable you to identify which of the components are crucial for the growth and productivity of the cell. 
3. Optimize the concentrations of the crucial components to maximize cell growth and productivity. 
4. Repeat the experiment to validate the optimal conditions you’ve identified and confirm that you obtain the same results. 
5. Scale up the process from laboratory to industrial scale, while making sure that you maintain the optimal growth and productivity conditions throughout. 
6. Monitor the performance continuously and adjust the media components as and when needed to ensure continued optimal growth and productivity.