What are the steps of tissue culture?

The process of tissue culturing can be divided into following steps:

• Step 1. Preparation of culture medium – contains a mixture of macro and micro nutrients, vitamins, hormones, nitrogen supplements, amino acids, growth regulators and solidifying agents.
• Step 2. Inoculation of explant – during this step the cultivated explant is sterilized and then carefully transferred to a suitable container and placed on the nutrient medium
• Step 3. Incubation of culture – prepared plant tissue cultures placed in a plant growth chamber or an incubator at 25 – 30°C to support growth.
• Step 4. Sub-culturing – to maintain adequate conditions for growth and development explants are divided and transferred to new media.
• Step 5. Transplantation – plants are acclimatized and then transplanted to soil