AAT Bioquest

What is a secondary culture?

Posted December 14, 2020


A secondary culture or cell line is a primary culture that has been sub-cultured or passaged. When a primary culture reaches confluency it must be removed from its current medium and transferred to a fresh growth medium. This ensures that the cells stay at an optimal density for continued growth and proliferation. Due to the providing of survival factors continuously, cells can acquire an indefinite lifespan.

Additional resources

Cell Proliferation Assays

Cell Sample Preparation