When should I use 1kb DNA ladder? | AAT Bioquest

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When should I use 1kb DNA ladder?

Posted May 2, 2023

Agarose gel electrophoresis DNA and RNA Gel Electrophoresis Nucleic Acids Nucleus polyacrylamide gel dna ladder

Answer

1kb DNA ladder consists of 13 double-stranded, blunt-end fragments. 1 kb DNA ladder is recommended for determining the size of double-stranded DNA in the range of 250 bp (base points) to 10,000 bp on agarose gels.

Additional resources

Assembly of Long Adapter Single Strands Oligonucleotide (LASSO) probes for massive parallel capture of kilobase size DNA targets DNA & RNA Markers and Ladders ReadiUse™ 1 Kb Plus DNA Ladder

How is a DNA ladder used in gel electrophoresis?Which technique is used to visualize DNA following a PCR reaction?How is a DNA ladder visualized in gel electrophoresis?How does the size of the DNA fragment affect its movement or migration through the agarose gel during electrophoresis?What are the limitations of in-gel staining?