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When should I use qPCR for DNA library normalization?
Posted June 19, 2024
DNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Sanger Sequencinggene sequencing
Answer

Use qPCR for DNA library normalization in these situations: 

  • Your samples are valuable, available in limited quality, or originate from varied sources
  • You are looking for the ultimate accuracy for normalization with no variations in quantitation and read depth
  • Ensuring a minimum target read depth is of utmost importance
Additional resources
Comparison between magnetic bead and qPCR library normalisation methods for forensic MPS genotyping Real-Time PCR (qPCR) Helixyte™ Green *20X Aqueous PCR Solution*

What is the typical workflow of DNA quantification and normalization?When should I use dPCR to quantify NGS libraries?What are the advantages of second generation NGS?What are the similarities between primers and promotors?What's the significance of reference genes?