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What is the typical workflow of DNA quantification and normalization?
Posted June 19, 2024
DNA and RNA QuantitationPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Sanger Sequencinggene sequencing
Answer

The typical workflow of DNA quantification and normalization can be encapsulated in 4 steps: 

  1. Set up quantification assay
  2. Determine DNA concentration
  3. Calculate a dilution scheme with the aim of achieving the target concentration for each well
  4. Dilute DNA samples to the desired concentration
Additional resources
The Illumina Sequencing Protocol and the NovaSeq 6000 System DNA and RNA Quantitation DNA Concentration Calculator Helixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*

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