Where is pyruvate found in the cell?

Pyruvate is formed in the cytosol by the process of glycolysis. The pyruvate is then transported into the mitochondria for energy synthesis. In prokaryotes, pyruvate oxidation occurs in the cytoplasm since they lack a nucleus. Pyruvate oxidation yields acetyl CoA, which acts as the energy source for the TSA cycle in the next stage of cellular respiration.