Why is DMSO added in MTT assay? | AAT Bioquest

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Why is DMSO added in MTT assay?

Posted October 26, 2022

Bioconjugation Cell Cycle Assays Cell Proliferation Assays Cell Structures and Organelles Cell Viability Assays Nucleus

Answer

DMSO is added toMTT assays at the end of the experiment to dissolve the formazan crystals that were created during the experiment. If DMSO is not added and the formazan crystals are not dissolved, this would cause significant changes in the absorbance spectrum due to the sodium bicarbonate that would still be there.

Additional resources

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