Cell Meter™ BX650 fixable viability dye
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 844.83 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 518 |
Excitation (nm) | 518 |
Emission (nm) | 654 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | ![]() ![]() |
See also: Cell Viability Assays, Cellular Processes, Flow Cytometry Reagents, Fluorescence Activated Cell Sorting (FACS)
Molecular weight 844.83 | Absorbance (nm) 518 | Excitation (nm) 518 | Emission (nm) 654 |
Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations. Cell Meter™ fixable viability dyes are a large family of cell-impermeable fluorescent viability dyes that are optimized to match the major excitation lasers of common flow cytometers, such as 350, 405, 488, 633 and 647 nm. These dyes are impermeant to live cells but permeant to cells with compromised membranes. They irreversibly react with amine- and thiol-containing proteins and other cellular components. Since dead or fixed cells with a compromised membrane more readily react with Cell Meter™ fixable cell stains, thus stain brighter than live cells with an intact membrane, these dyes can be used to assess live vs. dead status of mammalian cells. There are a few factors to be considered when using these dyes, e.g., the titration of each dye to ensure that live cells have minimal to no staining. Cell Meter™ BX650 fixable cell stain is optimized to be excited with the blue laser at 488 nm with emission at 650 nm. Compared to other commercially similar viability dyes, this fixable viability dye is much more robust and stable.
Platform
Flow cytometer
Excitation | 488 nm laser |
Emission | 695/40 nm filter |
Instrument specification(s) | PerCP channel |
Fluorescence microscope
Excitation | TexasRed filter set |
Emission | TexasRed filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
AT A GLANCE
Protocol summary
- Prepare samples in HHBS (0.5 mL/assay)
- Add Cell Meter™ BX650 to the cell suspension
- Stain the cells at room temperature for 20 - 60 minutes
- Wash the cells
- Fix the cells (optional)
- Examine the sample with flow cytometer using 695/40 nm filter (PerCP channel) and/or fluorescence microscope using Texas Red filter set
Important
Thaw at room temperature before starting the experiment.PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.
Note The unused Cell Meter™ BX650 stock solution should be divided into single use aliquots and stored at -20 °C. Avoid repeated freeze/thaw cycles.
Cell Meter™ BX650 stock solution (500X)
Add 200 µL of DMSO into the Cell Meter™ BX650 vial to make 500X stock solution.Note The unused Cell Meter™ BX650 stock solution should be divided into single use aliquots and stored at -20 °C. Avoid repeated freeze/thaw cycles.
SAMPLE EXPERIMENTAL PROTOCOL
- Treat cells as desired.
- Wash cells once with HHBS or the azide- and serum/protein-free buffer of your choice.
- Resuspend cells at 5 - 10 × 106/mL in HHBS or in the azide- and serum/protein-free buffer of your choice.
- Add 1 µL of 500X Cell Meter™ BX650 stock solution to 0.5 mL of cells/assay and mix it well.
- Incubate at room temperature, 5 % CO2 incubator for 20 - 60 minutes, protected from light.
Note The optimal stain concentrations and incubation time should be experimentally determined for different cell lines. - Wash cells twice and resuspend cells with HHBS or the buffer of your choice.
- Fix cells as desired (optional).
- Analyze cells with a flow cytometer using 695/40 nm filter (PerCP channel) and/or fluorescence microscope using Texas Red filter set.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cell Meter™ BX650 fixable viability dye to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 118.367 µL | 591.835 µL | 1.184 mL | 5.918 mL | 11.837 mL |
5 mM | 23.673 µL | 118.367 µL | 236.734 µL | 1.184 mL | 2.367 mL |
10 mM | 11.837 µL | 59.184 µL | 118.367 µL | 591.835 µL | 1.184 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 518 |
Excitation (nm) | 518 |
Emission (nm) | 654 |
Product Family
Name | Excitation (nm) | Emission (nm) |
Cell Meter™ BX520 fixable viability dye | 491 | 516 |
Cell Meter™ BX590 fixable viability dye | 492 | 579 |
Cell Meter™ IX830 fixable viability dye | 811 | 822 |
Cell Meter™ RX660 fixable viability dye | 649 | 664 |
Cell Meter™ RX700 fixable viability dye | 690 | 713 |
Cell Meter™ RX780 fixable viability dye | 629 | 767 |
Cell Meter™ VX450 fixable viability dye | 406 | 445 |
Cell Meter™ VX500 fixable viability dye | 433 | 498 |
Images

Figure 1. Detection of Jurkat cell viability by Cell Meter™ fixable viability dye. Jurkat cells were treated and stained with Cell Meter™ BX650 (Cat#22520), and then fixed in 3.7% formaldehyde and analyzed by flow cytometry. The dead cell population (Blue peak) is easily distinguished from the live cell population (Red peak) with PerCP channel, and nearly identical results were obtained before and after fixation.
References
View all 50 references: Citation Explorer
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Application notes
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