Name: Cell Meter™ BX650 fixable viability dye
Brand: AAT Bioquest
SKU: 22520
Price: 226 USD
Availability: InStock

Cell Meter™ BX650 fixable viability dye

Cell Meter™ BX650 Fixable Viability Dye is a fluorimetric reagent for distinguishing live and dead mammalian cells based on membrane integrity.

Detection Principle: Uses BX650 fluorescence to distinguish live and dead cells.
Signal Advantage: Provides robust, stable fluorescence retained after fixation.
Reaction Chemistry: Irreversibly binds amine- and thiol-containing cellular components.
Instrument Compatibility: Compatible with 488 nm laser flow cytometry (PerCP channel).
Applications: Identifies and excludes dead cells during flow cytometric analysis.

Detection of Jurkat cell viability by Cell Meter™ fixable viability dye. Jurkat cells were treated and stained with Cell Meter™ BX650 (Cat#22520), and then fixed in 3.7% formaldehyde and analyzed by flow cytometry.  The dead cell population (Blue peak)  is easily distinguished from the live cell population (Red peak)  with PerCP channel, and nearly identical results were obtained before and after fixation.

Protocol

SDS

Catalog	Size	Price	Quantity
22520	200 Tests	Price

Overview

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations. Cell Meter™ fixable viability dyes are a large family of cell-impermeable fluorescent viability dyes that are optimized to match the major excitation lasers of common flow cytometers, such as 350, 405, 488, 633 and 647 nm. These dyes are impermeant to live cells but permeant to cells with compromised membranes. They irreversibly react with amine- and thiol-containing proteins and other cellular components. Since dead or fixed cells with a compromised membrane more readily react with Cell Meter™ fixable cell stains, thus stain brighter than live cells with an intact membrane, these dyes can be used to assess live vs. dead status of mammalian cells. There are a few factors to be considered when using these dyes, e.g., the titration of each dye to ensure that live cells have minimal to no staining. Cell Meter™ BX650 fixable cell stain is optimized to be excited with the blue laser at 488 nm with emission at 650 nm. Compared to other commercially similar viability dyes, this fixable viability dye is much more robust and stable.

References

View all 50 references: Citation Explorer

Fluorescence-based method is more accurate than counting-based methods for plotting growth curves of adherent cells.

Authors:

Pereira, Túlio Felipe and Levin, Gabriel and DeOcesano-Pereira, Carlos and Caodaglio, Amanda Schiersner and Fujita, André and Tonso, Aldo and Sogayar, Mari Cleide

Journal:

BMC research notes (2020): 57

[Immune function of myeloid-derived suppressor cells and its mechanism in obstructive sleep apnea syndrome].

Authors:

Chen, S W and Li, J and Xiang, B and Xu, S J and Wang, L

Journal:

Zhonghua yi xue za zhi (2020): 295-300

[Effects of skin γδ T lymphocytes on wound healing of mice through regulating proliferation and differentiation of mice epidermal cells].

Authors:

Zhu, H J and Li, Y S and Wang, Y P and Hu, X H and Zhang, X R and Qiu, L and He, W F and Luo, G X

Journal:

Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns (2019): 298-307

CFDA-SE Combined with MACSiBeads™ Particles to Evaluate the Inhibitory Effect of Treg Cells in vitro.

Authors:

Ren, Qingqi and Jiang, Chunlin and Liu, Jikui

Journal:

Annals of clinical and laboratory science (2019): 740-747

Tracking keratinocytes and melanocytes using carboxyfluorescein hydroxysuccinimidyl ester staining.

Authors:

Lönnqvist, Susanna and Junker, Johan P E and Sedell, Maria and Nyman, Erika and Kratz, Gunnar

Journal:

PloS one (2019): e0221878

Physical properties

Solvent

DMSO

Spectral properties

Absorbance (nm)	518
Excitation (nm)	518
Emission (nm)	654

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	695/40 nm filter
Instrument specification(s)	PerCP channel

Fluorescence microscope
Excitation	TexasRed filter set
Emission	TexasRed filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on November 15, 2026