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Why is lysis important in DNA extraction?
Posted December 22, 2023
Buffer Preparations and RecipesDialysisExtractioncell lysis
Answer

Lysis is important in DNA extraction because it is the first of three steps. When lysis occurs, the nucleus and the cell are split open, releasing DNA and proteins. This process uses mechanical disruption, enzymes, and detergents such as Proteinase K to dissolve the cellular proteins and free DNA.  

Additional resources
DNA, RNA, and Protein Extraction: The Past and The PresentCell Lysis, Dialysis & Desalting Reagents Buffer Preparations and Recipes ReadiUse™ mammalian cell lysis buffer *5X*

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