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What is the best method for permeabilizing cells?
Posted May 9, 2020
Antibodies and Proteomics Chemical ReagentsBuffer Preparations and RecipesCellular Structures and OrganellesPBSPermeabilizationbuffercell lysisdetergent
Answer

Good options for permeabilizing cells are 0.1-0.2% saponin, Tween-20, or TritonX-100 in PBS.

Additional resources
ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*Tween® 20 Detergent PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe

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