Why is sample preparation important for NGS?

To ensure accurate and reliable results, samples for NGS experiments must undergo specific treatment and preparation before analysis. This not only helps to prevent contamination but also enhances accuracy and minimizes biases in the sequencing data. The specific considerations vary based on the sample type and the NGS platform being used. Sample preparation has become a critical aspect of NGS, as any mistakes in the preparation process can significantly impact the success of sequencing experiments. In specific, inadequate preparation can compromise subsequent processes like library construction, validation, sequencing and data analysis.