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RNA Concentration Calculator

The concentration of RNA in solution can be determined by substituting the molecular weight, extinction coefficient and λ max into a derived form of the Beer - Lambert Law. A substance's λmax is the wavelength at which it experiences the strongest absorbance. For RNA, this wavelength is 260 nm.

The absorbance at λmax can be measured using a spectrophotometer. There are some important things to keep in mind when measuring 260 absorbance:
  1. The RNA should be well-dissolved in solution. RNA precipitation will cause inaccuracies in concentration calculations.
  2. The absorbance reading should not exceed the maximum detection limit of the instrumentation. This can be identified by a plateau in the absorbance spectrum. If the absorbance spectrum plateaus, dilute the sample and try again.
  3. This calculator assumes a 1 cm pathlength for instrumentation. Standard cuvettes for spectrophotometers are typically 1 cm. If a different pathlength is used, value should be corrected during data entry.

How to use this tool

  1. Select the RNA type. Or select "custom sequence" to enter the nucleic acid sequence.

  2. Enter the absorbance at λmaxFor a typical nucleic acid sequence, λmax is 260 nm. However, this value may change based on the sequence.Please use the maximum absorbance as indicated by the spectrophotometric reading (ie. highest peak).

  3. If the absorbance is obtained with a diluted sample, enter the dilution factor in order to determine the concentration of the original sample.

  4. If pathlength differs from 1 cm, enter corrected value into pathlength textbox.

  5. Press calculate to display the concentration.

Data Entry

RNA
Absorbance at λmax
Dilution factor (optional)
Pathlength cm
Calculate



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References

This online tool may be cited as follows

MLA

"Quest Calculate™ RNA Concentration Calculator." AAT Bioquest, Inc.23 Sep2022https://www.aatbio.com/tools/calculate-RNA-concentration.

APA

AAT Bioquest, Inc. (2022September 23). Quest Calculate™ RNA Concentration Calculator. AAT Bioquest. https://www.aatbio.com/tools/calculate-RNA-concentration.