Actinomycin D

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1255.42
Solvent	DMSO

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Actinomycin D to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	79.655 µL	398.273 µL	796.546 µL	3.983 mL	7.965 mL
5 mM	15.931 µL	79.655 µL	159.309 µL	796.546 µL	1.593 mL
10 mM	7.965 µL	39.827 µL	79.655 µL	398.273 µL	796.546 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
D-Aminoluciferin	362	499	18000

Images

Figure 1. Chemical structure for Actinomycin D

Citations

View all 12 citations: Citation Explorer

Circ\_0044235 regulates the development of osteoarthritis by the modulation of miR-375/PIK3R3 axis
Authors: Qian, Wenjie and Mei, Kai and Zhu, Lei and Chu, Ying and Lv, Jinpeng and Yun, Changjun
Journal: Journal of Orthopaedic Surgery and Research (2024): 241

HBV DNA polymerase upregulates the transcription of PD-L1 and suppresses T cell activity in hepatocellular carcinoma
Authors: Jia, Yan and Zhao, Jianing and Wang, Chunqing and Meng, Jing and Zhao, Liqing and Yang, Hongwei and Zhao, Xiaoqing
Journal: Journal of Translational Medicine (2024): 1--17

N6-methyladenosine-regulated ADIRF impairs lung adenocarcinoma metastasis and serves as a potential prognostic biomarker
Authors: Teng, Yin and Zhao, Xiaohan and Xi, Yang and Fu, Ninghua
Journal: Cancer Biology \& Therapy (2023): 2249173

METTL3-mediated m6A methylation orchestrates mRNA stability and dsRNA contents to equilibrate $\gamma$$\delta$ T1 and $\gamma$$\delta$ T17 cells
Authors: Xiao, Zhiqiang and Wang, Shanshan and Tian, Yixia and Lv, Wenkai and Sheng, Hao and Zhan, Mingjie and Huang, Qiongxiao and Zhang, Zhanpeng and Zhu, Leqing and Zhu, Chuyun and others,
Journal: Cell Reports (2023)

METTL3 regulates N6-methyladenosine modification of ANGPTL3 mRNA and potentiates malignant progression of stomach adenocarcinoma
Authors: Zhang, Zhijin and Fu, Jun and Zhang, Yuhao and Qin, Xianju and Wang, Yuexia and Xing, Chungen
Journal: BMC gastroenterology (2023): 1--14

MEX3B inhibits collagen production in eosinophilic nasal polyps by downregulating epithelial cell TGFBR3 mRNA stability
Authors: Liu, Jin-Xin and Ao-Nan, Chen and Yu, Qihong and Shi, Ke-Tai and Liu, Yi-Bo and Guo, Cui-Lian and Wang, Zhe-Zheng and Yao, Yin and Pan, Li and Lu, Xiang and others,
Journal: JCI insight (2023)

Knockdown of METTL16 disrupts learning and memory by reducing the stability of MAT2A mRNA
Authors: Zhang, Runjiao and Zhang, Yizhou and Guo, Fangzhen and Huang, Guannan and Zhao, Yan and Chen, Bingyu and Wang, Chang and Cui, Chengran and Shi, Yichun and Li, Sha and others,
Journal: Cell death discovery (2022): 1--14

Exosomal circZFR promotes colorectal cancer progression via interacting with BCLAF1 and regulating the miR-3127-5p/RTKN2 axis
Authors: Chen, Jiaxin and Xu, Jianbin and Wang, Huijuan and Chen, Engeng and Meng, Qing and Wang, Jiawei and Zhou, Wei and Shan, Ge and Ju, Zhenyu and Song, Zhangfa
Journal: (2022)

CircRNA\_OTUD7A upregulates FOXP1 expression to facilitate the progression of diffuse large B-cell lymphoma via acting as a sponge of miR-431-5p
Authors: Liu, Wei and Lei, Lei and Liu, Xiaoying and Ye, Suiyan
Journal: Genes \& Genomics (2021): 653--667

Circ-KIAA0907 inhibits the progression of oral squamous cell carcinoma by regulating the miR-96-5p/UNC13C axis
Authors: Dong, Wenjie and Zhao, Lei and Zhang, Shiyang and Zhang, Shijie and Si, Hongyun
Journal: World Journal of Surgical Oncology (2021): 1--13

References

View all 66 references: Citation Explorer

Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2-producer
Authors: Kurosawa K, Bui VP, VanEssendelft JL, Willis LB, Lessard PA, Ghiviriga I, Samb and an TG, Rha CK, Sinskey AJ.
Journal: Appl Microbiol Biotechnol (2006): 145

Downregulation of inhibitor of apoptosis proteins in apoptotic human chondrocytes treated with tumor necrosis factor-alpha and actinomycin D
Authors: Yoshimura F, Kanno H, Uzuki M, Tajima K, Shimamura T, Sawai T.
Journal: Osteoarthritis Cartilage (2006): 435

Anti-apoptotic effect of hepatocyte growth factor from actinomycin D in hepatocyte-derived HL7702 cells is associated with activation of PI3K/Akt signaling
Authors: Li W, Cai S, Cai L, Li X.
Journal: Toxicol Lett (2006): 142

Carbon monoxide protects hepatocytes from TNF-alpha/Actinomycin D by inhibition of the caspase-8-mediated apoptotic pathway
Authors: Kim HS, Loughran PA, Kim PK, Billiar TR, Zuckerbraun BS.
Journal: Biochem Biophys Res Commun (2006): 1172

Single-dose actinomycin D: Efficacy in the prophylaxis of postmolar gestational trophoblastic neoplasia in adolescents with high-risk hydatidiform mole
Authors: Uberti EM, Diestel MC, Guimaraes FE, De Napoli G, Schmid H.
Journal: Gynecol Oncol (2006): 325

Prompt non-stacking binding of actinomycin D to hairpin oligonucleotide HP1 and slow redistribution from HP1 to DNA
Authors: Vekshin N, Kovalev A.
Journal: J Biochem (Tokyo) (2006): 185

Evaluation of an actinomycin-D-containing combination chemotherapy protocol with extended maintenance therapy for canine lymphoma
Authors: Siedlecki CT, Kass PH, Jakubiak MJ, Dank G, Lyons J, Kent MS.
Journal: Can Vet J (2006): 52

Dexamethasone, melphalan, actinomycin D, cytosine arabinoside (DMAC) protocol for dogs with relapsed lymphoma
Authors: Alvarez FJ, Kisseberth WC, Gallant SL, Couto CG.
Journal: J Vet Intern Med (2006): 1178

Expression of cell kinetics and death during monocyte-macrophage differentiation: effects of Actinomycin D and Vinblastine treatments
Authors: Spano A, Monaco G, Barni S, Sciola L.
Journal: Histochem Cell Biol. (2006)

Binding of actinomycin C1 (D) and actinomin to base-modified oligonucleotide duplexes with parallel chain orientation
Authors: Li H, Peng X, Leonard P, Seela F.
Journal: Bioorg Med Chem (2006): 4089