Cal-520® amine

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	909.68
Solvent	DMSO

Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520® amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	109.929 µL	549.644 µL	1.099 mL	5.496 mL	10.993 mL
5 mM	21.986 µL	109.929 µL	219.858 µL	1.099 mL	2.199 mL
10 mM	10.993 µL	54.964 µL	109.929 µL	549.644 µL	1.099 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75¹

Product Family

Name	Excitation (nm)	Emission (nm)	Quantum yield
Cal-520®, AM	492	515	0.75¹

Images

Figure 1. Cal-520 amine is an excellent building block that can be readily used to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target. It can be conjugated to a carboxy group via activated esters or EDC coupling chemistry.

References

View all 50 references: Citation Explorer

Metformin reverses oxidative stress‑induced mitochondrial dysfunction in pre‑osteoblasts via the EGFR/GSK‑3β/calcium pathway.
Authors: Cao, Fangming and Yang, Keda and Qiu, Shui and Li, Jie and Jiang, Wen and Tao, Lin and Zhu, Yue
Journal: International journal of molecular medicine (2023)

Piezo1 channel activation stimulates ATP production through enhancing mitochondrial respiration and glycolysis in vascular endothelial cells.
Authors: Jiang, Man and Zhang, Yi-Xin and Bu, Wen-Jie and Li, Ping and Chen, Jia-Hui and Cao, Ming and Dong, Yan-Chao and Sun, Zhi-Jie and Dong, De-Li
Journal: British journal of pharmacology (2023): 1862-1877

Pterosin sesquiterpenoids from Pteris laeta Wall. ex Ettingsh. protect cells from glutamate excitotoxicity by modulating mitochondrial signals.
Authors: Cheng, Aifang and Zhang, Yan and Sun, Jin and Huang, Duli and Sulaiman, Jordy Evan and Huang, Xin and Wu, Long and Ye, Wenkang and Wu, Chuanhai and Lam, Henry and Shi, Yusheng and Qian, Pei-Yuan
Journal: Journal of ethnopharmacology (2023): 116308

Live Imaging of CAR T Cell Ca2+ Signals in Tumor Slices Using Confocal Microscopy.
Authors: Espie, David and Barrin, Sarah and Rajnpreht, Irena and Vimeux, Lene and Donnadieu, Emmanuel
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 453-462

Aluminum alters excitability by inhibiting calcium, sodium, and potassium currents in bovine chromaffin cells.
Authors: Baraibar, Andrés M and de Pascual, Ricardo and Carretero, Victoria Jiménez and Liccardi, Ninfa and Juárez, Natalia Hernández and Hernández-Guijo, Jesús M
Journal: Journal of neurochemistry (2023): 162-176

Dibazol-induced relaxation of ophthalmic artery in C57BL/6J mice is correlated with the potency to inhibit voltage-gated Ca2+ channels.
Authors: Xu, Xinrong and Hou, Xiaomin and Xing, Ye and Feng, Tianfeng and Chai, Lina and Guo, Yunting and Chen, Liangjing and Shi, Yiwei and Qin, Xiaojiang
Journal: Experimental eye research (2023): 109468

Calcium Imaging of Non-adherent Cells.
Authors: Bye, Lydia J and Finol-Urdaneta, Rocio K and Adams, David J
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 361-369

Comparing the effects of chemical Ca2+ dyes and R-GECO on contractility and Ca2+ transients in adult and human iPSC cardiomyocytes.
Authors: Robinson, Paul and Sparrow, Alexander J and Psaras, Yiangos and Steeples, Violetta and Simon, Jillian N and Broyles, Connor N and Chang, Yu-Fen and Brook, Frances A and Wang, Ying-Jie and Blease, Andrew and Zhang, Xiaoyu and Abassi, Yama A and Geeves, Michael A and Toepfer, Christopher N and Watkins, Hugh and Redwood, Charles and Daniels, Matthew J
Journal: Journal of molecular and cellular cardiology (2023): 44-57

C-subfamily ATP binding cassette transporters extrude the calcium fluorescent probe fluo-4 from a cone photoreceptor cell line.
Authors: García-de-Diego, Antonio-Miguel
Journal: Naunyn-Schmiedeberg's archives of pharmacology (2023)

TRPA1 promotes the maturation of embryonic stem cell-derived cardiomyocytes by regulating mitochondrial biogenesis and dynamics.
Authors: Ding, Qianqian and Liu, Xianji and Qi, Yanxiang and Yao, Xiaoqiang and Tsang, Suk Ying
Journal: Stem cell research & therapy (2023): 158