Coelenterazine h *CAS#: 50909-86-9*

Coelenterazine h

CAS#: 50909-86-9

Calcium measurement is critical for numerous biological investigations. The aequorin complex comprises a 22 kD apoaequorin protein, molecular oxygen and the luminophore coelenterazine. When three Ca2+ ions bind to this complex, coelenterazine is oxidized to coelenteramide, with a concomitant release of carbon dioxide and blue light. The approximately third-power dependence of aequorin's bioluminescence on Ca2+ concentration allows the measurement of Ca2+ concentrations with a broad detection range from ~0.1 µM to >100 µM. Unlike fluorescent Ca2+ indicators, Ca2+-bound aequorin can be detected without illuminating the sample, thereby eliminating interference from autofluorescence. Native coelenterazine and its derivatives confer different Ca2+ affinities and spectral properties on the aequorin complex. Recombinant apoaequorin reconstituted with coelenterazine hcp is reported to have the best luminescence overall, with both a high quantum yield and a fast response time. However, intracellular reconstitution of aequorin from coelenterazine analogs can be relatively slow. Aequorins containing the cp, f or h form of coelenterazine exhibit 10-20 times stronger luminescence than that of apoaequorin reconstituted with native coelenterazine. Coelenterazines h and cp has been used in HTS screening assay for GPCRs.

Datasheet

Catalog	Size	Price
21153	250 ug	Price
21159	1 mg	Price
21165	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Ethanol needed to reconstitute specific mass of Coelenterazine h *CAS#: 50909-86-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	245.423 µL	1.227 mL	2.454 mL	12.271 mL	24.542 mL
5 mM	49.085 µL	245.423 µL	490.846 µL	2.454 mL	4.908 mL
10 mM	24.542 µL	122.711 µL	245.423 µL	1.227 mL	2.454 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	407.46 g/mol	=	mL	x	mM	=	mmol

Citations

View all 14 citations: Citation Explorer

Identification of compounds targeting porcine reproductive and respiratory syndrome virus nsp3 and evaluation of their antiviral efficacy

Authors:

He, Zhan and Nie, Zheng and Liao, Jiali and Liu, Min and Li, Fangfang and Chen, Binghua and Guo, Chunhe

Journal:

Virulence (2025): 2530176

In-silico predicted mouse melanopsins with blue spectral shifts deliver efficient subcellular signaling

Authors:

Wijayaratna, Dhanushan and Sacchetta, Filippo and Pedraza-Gonz{\'a}lez, Laura and Fanelli, Francesca and Sugihara, Tomohiro and Koyanagi, Mitsumasa and Piyawardana, Senuri and Ghotra, Kiran and Thotamune, Waruna and Terakita, Akihisa and others,

Journal:

Cell Communication and Signaling (2024): 1--22

Activation of dimerized BRS3-EP3 suppresses melanoma cell migration through coupling G$\alpha$s protein

Authors:

Wang, Zeyuan and Wu, Lehao and Guo, Miao and Zhu, Jianzheng and Zhao, Jiaqi and Wu, Yan and Xiao, Hua and Zhang, Yan

Journal:

Fundamental Research (2024)

Remdesivir inhibits Porcine epidemic diarrhea virus infection in vitro

Authors:

Huang, Zi-Xin and Zhou, Shu-Ting and Wang, Jing and Yang, Zhi-Biao and Wang, Zhe

Journal:

Heliyon (2023)

Wounding induces a peroxisomal H2O2 decrease via glycolate oxidase-catalase switch dependent on glutamate receptor-like channel-supported Ca2+ signaling in plants

Authors:

Li, Xiangyang and Chen, Linru and Zeng, Xiaoyue and Wu, Kaixin and Huang, Jiayu and Liao, Mengmeng and Xi, Yue and Zhu, Guohui and Zeng, Xiuying and Hou, Xuewen and others,

Journal:

The Plant Journal (2023)

References

View all 150 references: Citation Explorer

Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: catalytic properties for the oxidation of coelenterazine as an oxygenase

Authors:

Inouye S, Sasaki S.

Journal:

FEBS Lett (2006): 1977

Mitochondrial adaptations within chronically ischemic swine myocardium

Authors:

McFalls EO, Sluiter W, Schoonderwoerd K, Manintveld OC, Lamers JM, Bezstarosti K, van Beusekom HM, Sikora J, Ward HB, Merkus D, Duncker DJ.

Journal:

J Mol Cell Cardiol (2006): 980

Self-illuminating quantum dots light the way

Authors:

Frangioni JV., undefined

Journal:

Nat Biotechnol (2006): 326

Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development

Authors:

Cheung CY, Webb SE, Meng A, Miller AL.

Journal:

Int J Dev Biol (2006): 561

Expression, purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis

Authors:

Inouye S., undefined

Journal:

Protein Expr Purif. (2006)

Physical properties

Molecular weight	407.46
Solvent	Ethanol

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	50909-86-9

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