CytoTrace™ CM-DiI
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1051.51 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 148000 |
Excitation (nm) | 548 |
Emission (nm) | 563 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
See also: Plasma Membrane, Exosomes
Molecular weight 1051.51 | Extinction coefficient (cm -1 M -1) 148000 | Excitation (nm) 548 | Emission (nm) 563 |
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine iodide (CM-DiI) is a lipophilic carbocyanine dye that is commonly used for labeling cell membranes in various biological and neuroscientific research applications. It is a fluorescent dye that exhibits strong fluorescence properties when incorporated into cell membranes or lipid-containing structures. CM-DiI is widely used to label and trace cell membranes to study cell migration, cell tracking, and axonal projections in live and fixed tissues. Once incorporated into the cell membrane, CM-DiI tends to stay in place and does not rapidly diffuse within the membrane. This characteristic enables long-term imaging and tracing studies. In neuroscience, CM-DiI is particularly valuable for studying neural connections and pathways. Researchers can apply CM-DiI to specific regions of the brain or spinal cord and observe the labeled axons projecting to other regions. CM-DiI emits strong red fluorescence, making it easily detectable and suitable for fluorescence microscopy. CM-DiI is relatively stable, and its fluorescence is resistant to photobleaching, allowing for extended imaging sessions without significant loss of signal. It allows researchers to visualize and analyze labeled structures with high specificity. CM-DiI can be used in both cell cultures and living organisms, allowing researchers to study biological processes at various levels of complexity.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ CM-DiI to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 95.101 µL | 475.507 µL | 951.013 µL | 4.755 mL | 9.51 mL |
5 mM | 19.02 µL | 95.101 µL | 190.203 µL | 951.013 µL | 1.902 mL |
10 mM | 9.51 µL | 47.551 µL | 95.101 µL | 475.507 µL | 951.013 µL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 148000 |
Excitation (nm) | 548 |
Emission (nm) | 563 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
CytoTrace™ CM-DiD | 643 | 663 | 260000 |
Images
Figure 1. 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine iodide (CM-DiI) is a lipophilic carbocyanine dye that is commonly used for labeling cell membranes in various biological and neuroscientific research applications. It is a fluorescent dye that exhibits strong fluorescence properties when incorporated into cell membranes or lipid-containing structures.
References
View all 3 references: Citation Explorer
CLARITY-compatible lipophilic dyes for electrode marking and neuronal tracing.
Authors: Jensen, Kristian H R and Berg, Rune W
Journal: Scientific reports (2016): 32674
Authors: Jensen, Kristian H R and Berg, Rune W
Journal: Scientific reports (2016): 32674
Visualization of interstitial cells of Cajal in living, intact tissues.
Authors: Hanani, M and Belzer, V and Rich, A and Faussone-Pellegrini, S M
Journal: Microscopy research and technique (1999): 336-43
Authors: Hanani, M and Belzer, V and Rich, A and Faussone-Pellegrini, S M
Journal: Microscopy research and technique (1999): 336-43
Visualization of interstitial cells of Cajal in the mouse colon by vital staining.
Authors: Hanani, M and Louzon, V and Miller, S M and Faussone-Pellegrini, M S
Journal: Cell and tissue research (1998): 275-82
Authors: Hanani, M and Louzon, V and Miller, S M and Faussone-Pellegrini, M S
Journal: Cell and tissue research (1998): 275-82
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
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What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?