Mag-520™ potassium salt
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 511.51 |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 507 |
Emission (nm) | 531 |
Storage, safety and handling
Intended use | Research Use Only (RUO) |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Mag-520™ AM |
Overview | SDSProtocol |
See also: Intracellular Ions
Molecular weight 511.51 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 507 | Emission (nm) 531 |
A vast majority of the existing magnesium ion indicators are based on tricarboxylate APTRA chelator derived from the popular tetracarboxylate BAPTA calcium ion chelator. They include mag-fura-2, mag-indo-1, mag-fluo-4 and mag-rhod-3. However, all of them have higher affinity for calcium than magnesium although they were designed for detecting magnesium ion. Mag-520™ is the first commercial magnesium indicator that has higher affinity for magnesium than calcium. Its significantly improved selectivity can be used for measuring magnesium ion with minimal interference from calcium ion compared to other commercial magnesium ion indicators such as the popular mag-fluo-4.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Mag-520™ potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 195.5 µL | 977.498 µL | 1.955 mL | 9.775 mL | 19.55 mL |
5 mM | 39.1 µL | 195.5 µL | 390.999 µL | 1.955 mL | 3.91 mL |
10 mM | 19.55 µL | 97.75 µL | 195.5 µL | 977.498 µL | 1.955 mL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 507 |
Emission (nm) | 531 |
Product Family
Name | Excitation (nm) | Emission (nm) | Quantum yield |
Cal-520®, potassium salt | 492 | 515 | 0.751 |
Calbryte™ 520, potassium salt | 493 | 515 | 0.751 |
Images
Citations
View all 13 citations: Citation Explorer
Fluorescence lifetime imaging of intracellular magnesium content in live cells
Authors: Sargenti, A., C and eo, A., Farruggia, G., D'Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.
Journal: Analyst (2019): 1876-1880
Authors: Sargenti, A., C and eo, A., Farruggia, G., D'Andrea, C., Cappadone, C., Malucelli, E., Valentini, G., Taroni, P., Iotti, S.
Journal: Analyst (2019): 1876-1880
Synthesis of a highly Mg(2+)-selective fluorescent probe and its application to quantifying and imaging total intracellular magnesium
Authors: Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.
Journal: Nat Protoc (2017): 461-471
Authors: Sargenti, A., Farruggia, G., Zaccheroni, N., Marraccini, C., Sgarzi, M., Cappadone, C., Malucelli, E., Procopio, A., Prodi, L., Lombardo, M., Iotti, S.
Journal: Nat Protoc (2017): 461-471
A novel fluorescent chemosensor allows the assessment of intracellular total magnesium in small samples
Authors: Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.
Journal: Analyst (2014): 1201-7
Authors: Sargenti, A., Farruggia, G., Malucelli, E., Cappadone, C., Merolle, L., Marraccini, C., Andreani, G., Prodi, L., Zaccheroni, N., Sgarzi, M., Trombini, C., Lombardo, M., Iotti, S.
Journal: Analyst (2014): 1201-7
Intracellular magnesium detection by fluorescent indicators
Authors: Trapani, V., Schweigel-Rontgen, M., Cittadini, A., Wolf, F. I.
Journal: Methods Enzymol (2012): 421-44
Authors: Trapani, V., Schweigel-Rontgen, M., Cittadini, A., Wolf, F. I.
Journal: Methods Enzymol (2012): 421-44
Development of fluorescent probes for intracellular magnesium measurement and their applications to analysis of cellular mechanism
Authors: Shindo, Y., Oka, K., Komatsu, H., Suzuki, K.
Journal: Tanpakushitsu Kakusan Koso (2007): 1552-7
Authors: Shindo, Y., Oka, K., Komatsu, H., Suzuki, K.
Journal: Tanpakushitsu Kakusan Koso (2007): 1552-7
Mitochondria are intracellular magnesium stores: investigation by simultaneous fluorescent imagings in PC12 cells
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kudo, S., Kitamura, Y., Suzuki, K., Oka, K.
Journal: Biochim Biophys Acta (2005): 19-28
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kudo, S., Kitamura, Y., Suzuki, K., Oka, K.
Journal: Biochim Biophys Acta (2005): 19-28
Design and synthesis of highly sensitive and selective fluorescein-derived magnesium fluorescent probes and application to intracellular 3D Mg2+ imaging
Authors: Komatsu, H., Iwasawa, N., Citterio, D., Suzuki, Y., Kubota, T., Tokuno, K., Kitamura, Y., Oka, K., Suzuki, K.
Journal: J Am Chem Soc (2004): 16353-60
Authors: Komatsu, H., Iwasawa, N., Citterio, D., Suzuki, Y., Kubota, T., Tokuno, K., Kitamura, Y., Oka, K., Suzuki, K.
Journal: J Am Chem Soc (2004): 16353-60
Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kitamura, Y., Suzuki, K., Oka, K.
Journal: J Am Coll Nutr (2004): 742S-4S
Authors: Kubota, T., Shindo, Y., Tokuno, K., Komatsu, H., Ogawa, H., Kitamura, Y., Suzuki, K., Oka, K.
Journal: J Am Coll Nutr (2004): 742S-4S
Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1
Authors: Morelle, B., Salmon, J. M., Vigo, J., Viallet, P.
Journal: Anal Biochem (1994): 170-6
Authors: Morelle, B., Salmon, J. M., Vigo, J., Viallet, P.
Journal: Anal Biochem (1994): 170-6
Fluorescent monitoring of Jurkatt cell intracellular magnesium during metabolic poisoning
Authors: Carroll, R. G., Thomas, J. M.
Journal: Shock (1994): 213-6
Authors: Carroll, R. G., Thomas, J. M.
Journal: Shock (1994): 213-6
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Can you provide a brief protocol on how to use coelenterazine h for small animal in vivo imaging?
Are there any substitutes for probenecid in calcium assays?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Can you provide a brief protocol on how to use coelenterazine h for small animal in vivo imaging?