mFluor™ UV 375 goat anti-mouse IgG (H+L) *Cross-Absorbed*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~150 kDa |
Solvent | Water |
Spectral properties
Absorbance (nm) | 342 |
Correction Factor (260 nm) | 0.099 |
Correction Factor (280 nm) | 0.138 |
Extinction coefficient (cm -1 M -1) | 300001 |
Excitation (nm) | 351 |
Emission (nm) | 387 |
Quantum yield | 0.941 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Alternative formats
mFluor™ UV 375 goat anti-mouse IgG (H+L) |
Overview | SDSProtocol |
Molecular weight ~150 kDa | Absorbance (nm) 342 | Correction Factor (260 nm) 0.099 | Correction Factor (280 nm) 0.138 | Extinction coefficient (cm -1 M -1) 300001 | Excitation (nm) 351 | Emission (nm) 387 | Quantum yield 0.941 |
mFluor™ UV375 goat anti-mouse conjugates are secondary antibodies designed for optimal performance in immunoassay applications, including flow cytometry, immunofluorescence, and confocal microscopy. These conjugates consist of goat-derived polyclonal antibodies with high affinity and specificity towards mouse IgG, conjugated to the bright and stable mFluor™ UV375 fluorochrome. This conjugation is optimized to ensure minimal non-specific binding and enhanced signal clarity, with rigorous purification steps to remove unconjugated components. Provided in a ready-to-use format with a recommended dilution range, the conjugate undergoes stringent quality control tests for performance and specificity. Its compatibility with a wide range of mouse primary antibodies and the exceptional contrast provided by mFluor™ UV375 fluorescence makes it a reliable tool for detecting diverse target antigens in multicolor staining protocols. mFluor™ UV375 is optimally excited by the UV laser and emits maximally at 387 nm. These affinity-purified goat anti-mouse secondary antibodies are valuable for their versatility and sensitivity, enabling efficient detection, sorting, or purification of specific targets through effective signal amplification in research applications. To minimize cross-reactivity, these goat anti-mouse IgG whole antibodies have been cross-adsorbed against human, horse, mouse, and bovine IgG.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 342 |
Correction Factor (260 nm) | 0.099 |
Correction Factor (280 nm) | 0.138 |
Extinction coefficient (cm -1 M -1) | 300001 |
Excitation (nm) | 351 |
Emission (nm) | 387 |
Quantum yield | 0.941 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ UV 375 goat anti-rabbit IgG (H+L) | 351 | 387 | 300001 | 0.941 | 0.099 | 0.138 |
mFluor™ UV 375 goat anti-rabbit IgG (H+L) *Cross-Absorbed* | 351 | 387 | 300001 | 0.941 | 0.099 | 0.138 |
References
View all 50 references: Citation Explorer
In-situ and amplification-free imaging of hERG ion channels at single-cell level using a unique core-molecule-shell-secondary antibody SERS nanoprobe.
Authors: Zhang, Wen-Shu and Liu, Xiao-Peng and Yue, Shuai and Wang, Ya-Ning and Wang, Yue and Xu, Zhang-Run
Journal: Talanta (2023): 123900
Authors: Zhang, Wen-Shu and Liu, Xiao-Peng and Yue, Shuai and Wang, Ya-Ning and Wang, Yue and Xu, Zhang-Run
Journal: Talanta (2023): 123900
Use of immunoglobulin replacement therapy in patients with secondary antibody deficiency in daily practice: a European expert Q&A-based review.
Authors: Cinetto, Francesco and Francisco, Isabel Esteves and Fenchel, Klaus and Scarpa, Riccardo and Montefusco, Vittorio and Pluta, Andrzej and Wolf, Hermann M
Journal: Expert review of hematology (2023): 237-243
Authors: Cinetto, Francesco and Francisco, Isabel Esteves and Fenchel, Klaus and Scarpa, Riccardo and Montefusco, Vittorio and Pluta, Andrzej and Wolf, Hermann M
Journal: Expert review of hematology (2023): 237-243
Recommendations for Management of Secondary Antibody Deficiency in Multiple Myeloma.
Authors: Giralt, Sergio and Jolles, Stephen and Kerre, Tessa and Lazarus, Hillard M and Mustafa, S Shahzad and Papanicolaou, Genovefa A and Ria, Roberto and Vinh, Donald C and Wingard, John R
Journal: Clinical lymphoma, myeloma & leukemia (2023): 719-732
Authors: Giralt, Sergio and Jolles, Stephen and Kerre, Tessa and Lazarus, Hillard M and Mustafa, S Shahzad and Papanicolaou, Genovefa A and Ria, Roberto and Vinh, Donald C and Wingard, John R
Journal: Clinical lymphoma, myeloma & leukemia (2023): 719-732
Clinical outcomes of immunoglobulin treatment for patients with secondary antibody deficiency: Data from the Ontario immunoglobulin treatment case registry.
Authors: Abadeh, Armin and Shehadeh, Sarah and Betschel, Stephen and Waserman, Susan and Cameron, Donald William and Cowan, Juthaporn
Journal: PloS one (2023): e0294408
Authors: Abadeh, Armin and Shehadeh, Sarah and Betschel, Stephen and Waserman, Susan and Cameron, Donald William and Cowan, Juthaporn
Journal: PloS one (2023): e0294408
Development of recombinant secondary antibody mimics (rSAMs) for immunoassays through genetic fusion of monomeric alkaline phosphatase with antibody binders.
Authors: Park, Jiyeon and Bae, Yoonji and Eom, Soomin and Choi, Yuha and Lee, Giwook and Kang, Sebyung
Journal: International journal of biological macromolecules (2023): 126299
Authors: Park, Jiyeon and Bae, Yoonji and Eom, Soomin and Choi, Yuha and Lee, Giwook and Kang, Sebyung
Journal: International journal of biological macromolecules (2023): 126299
Endogenous complement-activating IgM is not required for primary antibody responses but promotes plasma cell differentiation and secondary antibody responses to a large particulate antigen in mice.
Authors: Palm, Anna-Karin E and Westin, Annika and Ayranci, Diyar and Heyman, Birgitta
Journal: Frontiers in immunology (2023): 1323969
Authors: Palm, Anna-Karin E and Westin, Annika and Ayranci, Diyar and Heyman, Birgitta
Journal: Frontiers in immunology (2023): 1323969
Severe coronavirus HCoV-NL63 pneumonia in a patient receiving blinatumomab with secondary antibody deficiency in COVID-19 times.
Authors: Paredes-Amaya, Claudia C and Matta-Cortes, Lorena and Zea-Vera, Andrés Felipe
Journal: Germs (2022): 292-297
Authors: Paredes-Amaya, Claudia C and Matta-Cortes, Lorena and Zea-Vera, Andrés Felipe
Journal: Germs (2022): 292-297
Subcutaneous immunoglobulins in chronic lymphocytic leukemia with secondary antibody deficiency. A monocentric experience during Covid-19 pandemics.
Authors: Innocenti, Idanna and Tomasso, Annamaria and Benintende, Giulia and Autore, Francesco and Fresa, Alberto and Vuono, Florenzia and Stirparo, Luca and Galli, Eugenio and D'Arena, Giovanni and Sorà, Federica and Efremov, Dimitar and Laurenti, Luca
Journal: Hematological oncology (2022): 469-474
Authors: Innocenti, Idanna and Tomasso, Annamaria and Benintende, Giulia and Autore, Francesco and Fresa, Alberto and Vuono, Florenzia and Stirparo, Luca and Galli, Eugenio and D'Arena, Giovanni and Sorà, Federica and Efremov, Dimitar and Laurenti, Luca
Journal: Hematological oncology (2022): 469-474
[Immunglobulin Substitution Therapy in Hematological Patients with secondary Antibody Deficiency].
Authors: Braschler, Thomas R and Zeerleder, Sacha
Journal: Therapeutische Umschau. Revue therapeutique (2022): 295-300
Authors: Braschler, Thomas R and Zeerleder, Sacha
Journal: Therapeutische Umschau. Revue therapeutique (2022): 295-300
Immunoglobulin substitution in patients with secondary antibody deficiency in chronic lymphocytic leukemia and multiple myeloma: a representative analysis of guideline adherence and infections.
Authors: Link, Hartmut and Kerkmann, Markus and Holtmann, Laura and , and ,
Journal: Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer (2022): 5187-5200
Authors: Link, Hartmut and Kerkmann, Markus and Holtmann, Laura and , and ,
Journal: Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer (2022): 5187-5200