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mFluor™ Violet 545-streptavidin conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | ~52 kDa |
| Solvent | Water |
Spectral properties
| Absorbance (nm) | 398 |
| Correction Factor (260 nm) | 1.08 |
| Correction Factor (280 nm) | 0.496 |
| Extinction coefficient (cm -1 M -1) | 200001 |
| Excitation (nm) | 393 |
| Emission (nm) | 543 |
| Quantum yield | 0.151 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDS |
Molecular weight ~52 kDa | Absorbance (nm) 398 | Correction Factor (260 nm) 1.08 | Correction Factor (280 nm) 0.496 | Extinction coefficient (cm -1 M -1) 200001 | Excitation (nm) 393 | Emission (nm) 543 | Quantum yield 0.151 |
The mFluor™ Violet 545-streptavidin conjugate is a tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 545 fluorescent dyes. It has a strong binding affinity for biotin conjugates, making it useful in various biochemical applications for detecting specific proteins, protein motifs, nucleic acids, and other molecules. This concept is essential for workflows such as western blots, flow cytometry, imaging and microscopy, and microplate assays, as well as for purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 545 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. When excited by a violet laser, mFluor™ Violet 545 emits bright and photostable green fluorescence at 543 nm. mFluor™ Violet 545 streptavidin conjugates are particularly useful as second-step reagents for indirect immunofluorescent staining when used with biotinylated primary antibodies.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 398 |
| Correction Factor (260 nm) | 1.08 |
| Correction Factor (280 nm) | 0.496 |
| Extinction coefficient (cm -1 M -1) | 200001 |
| Excitation (nm) | 393 |
| Emission (nm) | 543 |
| Quantum yield | 0.151 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| mFluor™ Violet 450-streptavidin conjugate | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
| mFluor™ Violet 510-streptavidin conjugate | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
| mFluor™ Violet 540-streptavidin conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
| mFluor™ Violet 550-streptavidin conjugate | 527 | 550 | 900001 | 0.311 | 0.474 | 0.306 |
| mFluor™ Violet 590-streptavidin conjugate | 564 | 591 | 900001 | 0.221 | 0.632 | 0.329 |
| mFluor™ Violet 610-streptavidin conjugate | 594 | 612 | 900001 | 0.31 | 0.532 | 0.66 |
References
View all 22 references: Citation Explorer
Low Concentrations of Chlorhexidine Inhibit the Formation and Structural Integrity of Enzyme-Treated Multispecies Oral Biofilms.
Authors: Gränicher, Kay Andrin and Karygianni, Lamprini and Attin, Thomas and Thurnheer, Thomas
Journal: Frontiers in microbiology (2021): 741863
Authors: Gränicher, Kay Andrin and Karygianni, Lamprini and Attin, Thomas and Thurnheer, Thomas
Journal: Frontiers in microbiology (2021): 741863
Protein immobilization onto electrochemically synthesized CoFe nanowires.
Authors: Torati, Sri Ramulu and Reddy, Venu and Yoon, Seok Soo and Kim, CheolGi
Journal: International journal of nanomedicine (2015): 645-51
Authors: Torati, Sri Ramulu and Reddy, Venu and Yoon, Seok Soo and Kim, CheolGi
Journal: International journal of nanomedicine (2015): 645-51
In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.
Authors: Towner, Rheal A and Smith, Nataliya and Saunders, Debra and Henderson, Michael and Downum, Kristen and Lupu, Florea and Silasi-Mansat, Robert and Ramirez, Dario C and Gomez-Mejiba, Sandra E and Bonini, Marcelo G and Ehrenshaft, Marilyn and Mason, Ronald P
Journal: Diabetes (2012): 2405-13
Authors: Towner, Rheal A and Smith, Nataliya and Saunders, Debra and Henderson, Michael and Downum, Kristen and Lupu, Florea and Silasi-Mansat, Robert and Ramirez, Dario C and Gomez-Mejiba, Sandra E and Bonini, Marcelo G and Ehrenshaft, Marilyn and Mason, Ronald P
Journal: Diabetes (2012): 2405-13
Sequence-dependent fluorescence of cyanine dyes on microarrays.
Authors: Agbavwe, Christy and Somoza, Mark M
Journal: PloS one (2011): e22177
Authors: Agbavwe, Christy and Somoza, Mark M
Journal: PloS one (2011): e22177
Molecular MRI assessment of vascular endothelial growth factor receptor-2 in rat C6 gliomas.
Authors: He, Ting and Smith, Nataliya and Saunders, Debra and Doblas, Sabrina and Watanabe, Yasuko and Hoyle, Jessica and Silasi-Mansat, Robert and Lupu, Florea and Lerner, Megan and Brackett, Daniel J and Towner, Rheal A
Journal: Journal of cellular and molecular medicine (2011): 837-49
Authors: He, Ting and Smith, Nataliya and Saunders, Debra and Doblas, Sabrina and Watanabe, Yasuko and Hoyle, Jessica and Silasi-Mansat, Robert and Lupu, Florea and Lerner, Megan and Brackett, Daniel J and Towner, Rheal A
Journal: Journal of cellular and molecular medicine (2011): 837-49
Oligodendrocytes in mouse corpus callosum are coupled via gap junction channels formed by connexin47 and connexin32.
Authors: Maglione, Marta and Tress, Oliver and Haas, Brigitte and Karram, Khalad and Trotter, Jacqueline and Willecke, Klaus and Kettenmann, Helmut
Journal: Glia (2010): 1104-17
Authors: Maglione, Marta and Tress, Oliver and Haas, Brigitte and Karram, Khalad and Trotter, Jacqueline and Willecke, Klaus and Kettenmann, Helmut
Journal: Glia (2010): 1104-17
Development and clinical evaluation of a microarray for hepatitis C virus genotyping.
Authors: Park, Jae-Chan and Kim, Jong-Man and Kwon, Oh-Joong and Lee, Kyoung-Ryul and Chai, Young-Guy and Oh, Heung-Bum
Journal: Journal of virological methods (2010): 269-75
Authors: Park, Jae-Chan and Kim, Jong-Man and Kwon, Oh-Joong and Lee, Kyoung-Ryul and Chai, Young-Guy and Oh, Heung-Bum
Journal: Journal of virological methods (2010): 269-75
In vivo detection of inducible nitric oxide synthase in rodent gliomas.
Authors: Towner, Rheal A and Smith, Nataliya and Doblas, Sabrina and Garteiser, Philippe and Watanabe, Yasuko and He, Ting and Saunders, Debra and Herlea, Oana and Silasi-Mansat, Robert and Lupu, Florea
Journal: Free radical biology & medicine (2010): 691-703
Authors: Towner, Rheal A and Smith, Nataliya and Doblas, Sabrina and Garteiser, Philippe and Watanabe, Yasuko and He, Ting and Saunders, Debra and Herlea, Oana and Silasi-Mansat, Robert and Lupu, Florea
Journal: Free radical biology & medicine (2010): 691-703
SNPs detection by a single-strand specific nuclease on a PNA zip-code microarray.
Authors: Mun, Hyo Young and Girigoswami, Agnishwar and Jung, Cheulhee and Cho, Dae-Yeon and Park, Hyun Gyu
Journal: Biosensors & bioelectronics (2009): 1706-11
Authors: Mun, Hyo Young and Girigoswami, Agnishwar and Jung, Cheulhee and Cho, Dae-Yeon and Park, Hyun Gyu
Journal: Biosensors & bioelectronics (2009): 1706-11
[A novel protein microarray detection technique based on biotin-avidin conjugation probe].
Authors: Yu, Xiaobo and Zhao, Tianming and Sun, Zhidan and Yuan, Hongkun and He, Wei and Xu, Danke
Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (2008): 515-20
Authors: Yu, Xiaobo and Zhao, Tianming and Sun, Zhidan and Yuan, Hongkun and He, Wei and Xu, Danke
Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology (2008): 515-20