mFluor™ Violet 590-streptavidin conjugate
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | ~52 kDa |
Solvent | Water |
Spectral properties
Absorbance (nm) | 563 |
Correction Factor (260 nm) | 0.632 |
Correction Factor (280 nm) | 0.329 |
Extinction coefficient (cm -1 M -1) | 900001 |
Excitation (nm) | 564 |
Emission (nm) | 591 |
Quantum yield | 0.221 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDS |
Molecular weight ~52 kDa | Absorbance (nm) 563 | Correction Factor (260 nm) 0.632 | Correction Factor (280 nm) 0.329 | Extinction coefficient (cm -1 M -1) 900001 | Excitation (nm) 564 | Emission (nm) 591 | Quantum yield 0.221 |
mFluor™ Violet 590-streptavidin conjugate consists of the tetrameric biotin-binding protein (streptavidin) covalently labeled with mFluor™ Violet 590 fluorescent dyes. Its high affinity for biotin conjugates is leveraged across a wide range of biochemical applications for the specific detection of various proteins, protein motifs, nucleic acids, and other molecules. This concept is central to the workflow of several detection protocols, including western blots, flow cytometry, imaging and microscopy, and microplate assays, and is crucial in purification workflows aimed at targeted fractionation. The conjugation of mFluor™ Violet 590 with streptavidin is carefully optimized to maximize fluorescence intensity while preserving the inherent binding properties of streptavidin. Upon excitation by a violet laser, mFluor ™ Violet 590 exhibits a bright and photostable emission of orange fluorescence at 591 nm. mFluor™ Violet 590 streptavidin conjugates are highly effective as second-step reagents for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 563 |
Correction Factor (260 nm) | 0.632 |
Correction Factor (280 nm) | 0.329 |
Extinction coefficient (cm -1 M -1) | 900001 |
Excitation (nm) | 564 |
Emission (nm) | 591 |
Quantum yield | 0.221 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
mFluor™ Violet 450-streptavidin conjugate | 406 | 445 | 350001 | 0.811 | 0.338 | 0.078 |
mFluor™ Violet 510-streptavidin conjugate | 412 | 505 | 250001 | 0.861 | 0.464 | 0.366 |
mFluor™ Violet 540-streptavidin conjugate | 402 | 535 | 180001 | 0.211 | 1.326 | 0.543 |
mFluor™ Violet 545-streptavidin conjugate | 393 | 543 | 200001 | 0.151 | 1.08 | 0.496 |
mFluor™ Violet 550-streptavidin conjugate | 527 | 550 | 900001 | 0.311 | 0.474 | 0.306 |
mFluor™ Violet 610-streptavidin conjugate | 594 | 612 | 900001 | 0.31 | 0.532 | 0.66 |
References
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Authors: Gao, Jie and Huang, Yan Yun and Zhang, Yu Tong and DU, Xiang Xin and Zhang, Li Na and Hao, Na and Guo, Xia and Li, Jian Guo and Zhang, Yu
Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (2021): 445-448
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Authors: Mercier, Frederic and Douet, Vanessa
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Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1993): 627-30
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