Nonyl acridine orange *CAS 75168-11-5*
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Physical properties
Molecular weight | 472.5 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 496 |
Emission (nm) | 519 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Mitochondria
CAS 75168-11-5 | Molecular weight 472.5 | Excitation (nm) 496 | Emission (nm) 519 |
10-n-Nonyl acridine orange (NAO) is a mitochondrial-specific fluorochrome. In contrast with Rh 123, NAO accumulation in the cell does not seem to be driven by the proton-motrice force but does seem to be related to specific interactions with mitochondrial membrane proteins and/or lipids. NAO uptake is independent of mitochondrial transmembrane potential. It is used to examine the changes in the mitochondrial compartment during the transfer of articular chondrocytes from cartilage to the culture conditions, where Rh 123 proves changes in mitochondrial activity and/or biogenesis. It is used for distinguishing mitochondrial activity from biogenesis. NAO and Rhodamine 123 (Rh 123) is a good pair of dyes for studying mitochondrial functions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Nonyl acridine orange *CAS 75168-11-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 211.64 µL | 1.058 mL | 2.116 mL | 10.582 mL | 21.164 mL |
5 mM | 42.328 µL | 211.64 µL | 423.28 µL | 2.116 mL | 4.233 mL |
10 mM | 21.164 µL | 105.82 µL | 211.64 µL | 1.058 mL | 2.116 mL |
Molarity calculator
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References
View all 15 references: Citation Explorer
Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Determination of dipole moment in the ground and excited state by experimental and theoretical methods of N-nonyl acridine orange
Authors: Wiosetek-Reske AM, Wysocki S, Bak GW.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2005): 1172
Authors: Wiosetek-Reske AM, Wysocki S, Bak GW.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2005): 1172
Binding of 10-N-nonyl acridine orange to cardiolipin-deficient yeast cells: implications for assay of cardiolipin
Authors: Gohil VM, Gvozdenovic-Jeremic J, Schlame M, Greenberg ML.
Journal: Anal Biochem (2005): 350
Authors: Gohil VM, Gvozdenovic-Jeremic J, Schlame M, Greenberg ML.
Journal: Anal Biochem (2005): 350
Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different experimental models
Authors: Garcia Fern, undefined and ez MI, Ceccarelli D, Muscatello U.
Journal: Anal Biochem (2004): 174
Authors: Garcia Fern, undefined and ez MI, Ceccarelli D, Muscatello U.
Journal: Anal Biochem (2004): 174
Intracellular distribution of the fluorescent dye nonyl acridine orange responds to the mitochondrial membrane potential: implications for assays of cardiolipin and mitochondrial mass
Authors: Jacobson J, Duchen MR, Heales SJ.
Journal: J Neurochem (2002): 224
Authors: Jacobson J, Duchen MR, Heales SJ.
Journal: J Neurochem (2002): 224
Cardiolipin binds nonyl acridine orange by aggregating the dye at exposed hydrophobic domains on bilayer surfaces
Authors: Mileykovskaya E, Dowhan W, Birke RL, Zheng D, Lutterodt L, Haines TH.
Journal: FEBS Lett (2001): 187
Authors: Mileykovskaya E, Dowhan W, Birke RL, Zheng D, Lutterodt L, Haines TH.
Journal: FEBS Lett (2001): 187
Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green, and MitoTracker Green is affected by mitochondrial membrane potential altering drugs
Authors: Keij JF, Bell-Prince C, Steinkamp JA.
Journal: Cytometry (2000): 203
Authors: Keij JF, Bell-Prince C, Steinkamp JA.
Journal: Cytometry (2000): 203
Visualization of phospholipid domains in Escherichia coli by using the cardiolipin-specific fluorescent dye 10-N-nonyl acridine orange
Authors: Mileykovskaya E, Dowhan W.
Journal: J Bacteriol (2000): 1172
Authors: Mileykovskaya E, Dowhan W.
Journal: J Bacteriol (2000): 1172
Direct cardiolipin assay in yeast using the red fluorescence emission of 10-N-nonyl acridine orange
Authors: Gallet PF, Maftah A, Petit JM, Denis-Gay M, Julien R.
Journal: Eur J Biochem (1995): 113
Authors: Gallet PF, Maftah A, Petit JM, Denis-Gay M, Julien R.
Journal: Eur J Biochem (1995): 113
10N-nonyl acridine orange interacts with cardiolipin and allows the quantification of this phospholipid in isolated mitochondria
Authors: Petit JM, Maftah A, Ratinaud MH, Julien R.
Journal: Eur J Biochem (1992): 267
Authors: Petit JM, Maftah A, Ratinaud MH, Julien R.
Journal: Eur J Biochem (1992): 267
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
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What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?