Correction factors and critical values for iFluor®, Alexa Fluor®, and other commonly used dyes.

Name: Correction factors and critical values for iFluor®, Alexa Fluor®, and other commonly used dyes.
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Fluorophore ▲ ▼	Molecular Weight ▲ ▼	Ex (nm) ▲ ▼	Em (nm) ▲ ▼	ε¹ ▲ ▼	CF280² ▲ ▼	CF260³ ▲ ▼
iFluor® 350	749.85	345	450	20,000	0.23	0.83
iFluor® 405	755.58	403	427	37,000	0.77	0.48
iFluor® 430	688.79	433	498	40,000	0.3	0.68
iFluor® 440	692.83	434	480	40,000	0.229	0.352
iFluor® 450	603.69	451	502	40,000	0.27	0.45
iFluor® 460	793.99	468	493	80,000	0.46	0.98
iFluor® 488	945.07	491	516	75,000	0.11	0.21
iFluor® 514	1013.95	511	527	75,000	0.116	0.265
iFluor® 532	914.06	537	560	90,000	0.16	0.26
iFluor® 546	1145.41	541	557	100,000	0.15	0.25
iFluor® 555	1125.26	557	570	100,000	0.14	0.23
iFluor® 560	1257.45	560	571	120,000	0.069	0.0482
iFluor® 568	1173.46	568	587	100,000	0.15	0.34
iFluor® 570	881.07	560	571	120,000	0.069	0.048
iFluor® 594	1160.42	588	604	180,000	0.04	0.05
iFluor® 597	1058.29	598	618	100,000	0.514	0.335
iFluor® 610	1253.52	610	628	110,000	0.49	0.32
iFluor® 633	1249.58	640	654	250,000	0.044	0.062
iFluor® 647	1274.66	656	670	250,000	0.03	0.03
iFluor® 660	1281.66	663	678	250,000	0.08	0.07
iFluor® 670	923.15	671	682	200,000	0.033	0.03
iFluor® 680	957.17	684	701	220,000	0.094	0.097
iFluor® 690	1462.75	685	704	220,000	0.06	0.09
iFluor® 700	977.16	690	713	220,000	0.04	0.09
iFluor® 710	1206.39	717	739	190,000	0.07	0.12
iFluor® 720	1089.29	716	740	240,000	0.13	0.15
iFluor® 740	1551.19	742	764	225,000	0.16	0.16
iFluor® 750	1416.83	757	779	275,000	0.039	0.044
iFluor® 770	1082.27	777	797	250,000	0.08	0.09
iFluor® 780	1526.90	784	808	250,000	0.12	0.13
iFluor® 790	1768.30	787	812	250,000	0.09	0.1
iFluor® 800	1541.91	801	820	250,000	0.08	0.03
iFluor® 810	1576.03	811	822	250,000	0.15	0.09
iFluor® 820	1585.92	822	850	250,000	0.16	0.11
iFluor® 830	1527.88	830	867	-	-	-
iFluor® 840	1422	836	879	200,000	0.09	0.2
iFluor® 860	1571.90	853	878	250,000	0.14	0.1
iFluor® A7	1288.58	762	782	275,000	0.03	0.03
XFD350 Same Structure to Alexa Fluor® 350	410.35	343	439	19,000	0.19	0.25
XFD488 Same Structure to Alexa Fluor® 488	643.40	500	520	71,000	0.11	0.30
XFD514 Same Structure to Alexa Fluor® 514	916.07	518	543	80,000	0.18	0.31
XFD532 Same Structure to Alexa Fluor® 532	723.77	534	553	81,000	0.09	0.24
XFD546 Same Structure to Alexa Fluor® 546	1159.60	561	572	112,000	0.12	0.21
XFD555 Same Structure to Alexa Fluor® 555	∼1250	553	568	150,000	0.08	0.08
XFD568 Same Structure to Alexa Fluor® 568	791.80	579	603	91,300	0.46	0.45
XFD594 Same Structure to Alexa Fluor® 594	819.85	591	618	90,000	0.56	0.43
XFD647 Same Structure to Alexa Fluor® 647	1259.66	650	671	239,000	0.03	0.00
XFD680 Same Structure to Alexa Fluor® 680	∼1200	681	704	184,000	0.05	0.00
XFD700 Same Structure to Alexa Fluor® 700	∼1400	696	719	192,000	0.07	0.00
XFD750 Same Structure to Alexa Fluor® 750	∼1300	752	776	240,000	0.04	0.00
DyLight™ 350	∼874	353	432	15,000	0.144	-
DyLight™ 405	∼793	400	420	30,000	0.564	-
DyLight™ 488	∼1011	493	518	70,000	0.147	-
DyLight™ 550	∼1040	562	576	150,000	0.081	-
DyLight™ 594	∼1078	593	618	80,000	0.585	-
DyLight™ 633	∼1066	638	658	170,000	0.110	-
DyLight™ 650	∼1066	652	672	250,000	0.037	-
DyLight™ 680	∼950	682	715	140,000	0.128	-
DyLight™ 755	∼1092	755	776	220,000	0.030	-
DyLight™ 800	∼1050	770	794	270,000	0.045	-

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References

This online tool may be cited as follows

MLA	"Quest Database™ Correction factors and critical values for iFluor®, Alexa Fluor®, and other commonly used dyes.." AAT Bioquest, Inc., 13 May. 2025, https://www.aatbio.com/data-sets/correction-factors-and-critical-values-for-ifluor-alexa-fluor-and-other-commonly-used-dyes.
APA	AAT Bioquest, Inc. (2025, May 13). Quest Database™ Correction factors and critical values for iFluor®, Alexa Fluor®, and other commonly used dyes.. AAT Bioquest. https://www.aatbio.com/data-sets/correction-factors-and-critical-values-for-ifluor-alexa-fluor-and-other-commonly-used-dyes.
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Correction factors and critical values for iFluor®, Alexa Fluor®, and other commonly used dyes.

References

This online tool may be cited as follows

MLA

APA