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Intracellular Hydrogen Peroxide Probes
▲ ▼ | DCFH-DA ▲ ▼ | Dihydrorhodamine 123 ▲ ▼ | OxiVision™ Blue ▲ ▼ | OxiVision™ Green ▲ ▼ |
| Principle | Esterases cleave off diacetate group to yield DCFH which is oxidized by H2O2 to DCF and fluoresces green upon excitation | Oxidation by H2O2 yields rhodamine 123, which fluoresces blue upon excitation | OxiVision™ probes are oxidized by intracellular H2O2. Generates fluorescence upon excitation. | OxiVision™ probes are oxidized by intracellular H2O2. Generates fluorescence upon excitation. |
| Ex/Em (nm) | 504/525 | 507/527 | 405/450 | 497/516 |
| Filter Set | FITC | FITC | DAPI | FITC |
| Live Cells | Yes | Yes | Yes | Yes |
| Imaging | Yes | Yes | Yes | Yes |
| HCS | Yes | Yes | Yes | Yes |
| Flow Cytometry | Yes | Yes | Yes | Yes |
| Unit Size | 25 mg | 10 mg | Kit (100 Tests) | Kit (100 Tests) |
| Cat No. | 15204 | 15206 | 11504 (Imaging) 11505 (Flow) | 11503 (Imaging) 11506 (Flow) |
References
This online tool may be cited as follows
MLA | "Quest Database™ Intracellular Hydrogen Peroxide Probes." AAT Bioquest, Inc., 16 Aug. 2025, https://www.aatbio.com/data-sets/intracellular-hydrogen-peroxide-probes. | |
APA | AAT Bioquest, Inc. (2025, August 16). Quest Database™ Intracellular Hydrogen Peroxide Probes. AAT Bioquest. https://www.aatbio.com/data-sets/intracellular-hydrogen-peroxide-probes. | |
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