AzoDye-1 Succinimidyl Ester

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Physical properties

Molecular weight	601.62
Solvent	DMSO

Spectral properties

Absorbance (nm)

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Molecular weight

601.62

Absorbance (nm)

522

AzoDye-1 Succinimidyl Ester is an excellent building block for labeling amino-containing molecules. AzoDye-1 dyes are nearly non-fluorescent with moderate extinction coefficients. These dark quenchers are most typically used in Molecular Beacon probes in which FRET has minimal contribution to their fluorescence quenching, thus dependence on donor-acceptor spectral overlap is not significant as to other FRET probes such as FRET peptides. AzoDye-1 dyes can quench a variety of fluorophores, in particular, fluorescein derivatives such as FAM, HEX, TET, NED and JOE. This may reflect the AzoDye-1's ability to form stable, non-fluorescent complexes with a fluorophore.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of AzoDye-1 Succinimidyl Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	166.218 µL	831.089 µL	1.662 mL	8.311 mL	16.622 mL
5 mM	33.244 µL	166.218 µL	332.436 µL	1.662 mL	3.324 mL
10 mM	16.622 µL	83.109 µL	166.218 µL	831.089 µL	1.662 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)

522

Images

Figure 1. Chemical structure for AzoDye-1 Succinimidyl Ester.

References

View all 15 references: Citation Explorer

BHQ-Cyanine-Based "Off-On" Long-Circulating Assembly as a Ferroptosis Amplifier for Cancer Treatment: A Lipid-Peroxidation Burst Device.
Authors: Sang, Mangmang and Luo, Renjie and Bai, Yidan and Dou, Jun and Zhang, Zhongtao and Liu, Fulei and Feng, Feng and Liu, Wenyuan
Journal: ACS applied materials & interfaces (2019): 42873-42884

Förster Resonance Energy Transfer Mediated Photoluminescence Quenching in Stoichiometrically Assembled CdSe/ZnS Quantum Dot-Peptide Labeled Black Hole Quencher Conjugates for Matrix Metalloproteinase-2 Sensing.
Authors: Pillai, Sreenadh Sasidharan and Yukawa, Hiroshi and Onoshima, Daisuke and Biju, Vasudevanpillai and Baba, Yoshinobu
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2017): 137-142

The G-BHQ synergistic effect: Improved double quenching molecular beacons based on guanine and Black Hole Quencher for sensitive simultaneous detection of two DNAs.
Authors: Xiang, Dongshan and Li, Fengquan and Wu, Chenyi and Shi, Boan and Zhai, Kun
Journal: Talanta (2017): 289-294

One-pot Synthesis of Quencher Labeled Hairpin DNA-CdTe QDs Conjugate for Target DNA and Deoxyribonuclease I Detection.
Authors: Zhang, Qingfeng and Li, Juanmin and Zhang, Yunyi and Hu, Zhenzhen and Yu, Cong
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2016): 1035-1037

A Small-Molecule Inhibitor of Lin28.
Authors: Roos, Martina and Pradère, Ugo and Ngondo, Richard P and Behera, Alok and Allegrini, Sara and Civenni, Gianluca and Zagalak, Julian A and Marchand, Jean-Rémy and Menzi, Mirjam and Towbin, Harry and Scheuermann, Jörg and Neri, Dario and Caflisch, Amedeo and Catapano, Carlo V and Ciaudo, Constance and Hall, Jonathan
Journal: ACS chemical biology (2016): 2773-2781

Fluorescence Quenching of CdSe/ZnS Quantum Dots by Using Black Hole Quencher Molecules Intermediated With Peptide for Biosensing Application.
Authors: Pillai, Sreenadh Sasidharan and Yukawa, Hiroshi and Onoshima, Daisuke and Biju, Vasudevanpillai and Baba, Yoshinobu
Journal: Cell medicine (2015): 57-62

Real-time fluorescence assays to monitor duplex unwinding and ATPase activities of helicases.
Authors: Özeş, Ali R and Feoktistova, Kateryna and Avanzino, Brian C and Baldwin, Enoch P and Fraser, Christopher S
Journal: Nature protocols (2014): 1645-61

Optimal dye-quencher pairs for the design of an "activatable" nanoprobe for optical imaging.
Authors: Simard, Bryan and Tomanek, Boguslaw and van Veggel, Frank C J M and Abulrob, Abedelnasser
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2013): 1824-9

Universal dark quencher based on "clicked" spectrally distinct azo dyes.
Authors: Chevalier, Arnaud and Hardouin, Julie and Renard, Pierre-Yves and Romieu, Anthony
Journal: Organic letters (2013): 6082-5

Signal-amplification detection of small molecules by use of Mg2+-dependent DNAzyme.
Authors: Guo, Zhijun and Wang, Jiahai and Wang, Erkang
Journal: Analytical and bioanalytical chemistry (2013): 4051-7