ReadiCleave™ SSL biotin NHS ester

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	504.64
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

122266-55-1

Molecular weight

504.64

ReadiCleave™ SSL biotin NHS ester is a cleavable biotinylating reagent which can efficiently introduce a biotin moiety to amine-containing biomolecules. This biotin compound contains a cleavable linker that can be cleaved using reducing agents such as DTT or TCEP to remove the biotin label when needed.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ SSL biotin NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	198.161 µL	990.805 µL	1.982 mL	9.908 mL	19.816 mL
5 mM	39.632 µL	198.161 µL	396.322 µL	1.982 mL	3.963 mL
10 mM	19.816 µL	99.081 µL	198.161 µL	990.805 µL	1.982 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
ReadiCleave™ Cy5-SSL-NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
ReadiCleave™ XFD532 SSL-NHS ester	534	553	81000	0.61¹	0.24	0.09
ReadiCleave™ FAM SSL-NHS ester	491	516	73000	0.92	-	0.35

Images

Figure 1. Chemical structure for ReadiCleave™ SSL biotin NHS ester.

References

View all 48 references: Citation Explorer

In Situ Membrane Biotinylation Enables the Direct Labeling and Accurate Kinetic Analysis of Small Extracellular Vesicles in Circulation.
Authors: Yu, Zi-Li and Zhao, Yi and Miao, Fan and Wu, Min and Xia, Hou-Fu and Chen, Zhuo-Kun and Liu, Hai-Ming and Zhao, Yi-Fang and Chen, Gang
Journal: Analytical chemistry (2021): 10862-10870

Tris(hydroxymethyl)aminomethane Compatibility with N-Hydroxysuccinimide Ester Chemistry: Biotinylation of Peptides and Proteins in TRIS Buffer.
Authors: Kratzer, Ulrich and Sommersdorf, Cornelia and Maier, Sandra and Wagner, Teresa R and Templin, Markus and Joos, Thomas O and Rothbauer, Ulrich and Zeck, Anne and Poetz, Oliver
Journal: Bioconjugate chemistry (2021)

A Toolbox for Efficient Proximity-Dependent Biotinylation in Zebrafish Embryos.
Authors: Rosenthal, Shimon M and Misra, Tvisha and Abdouni, Hala and Branon, Tess C and Ting, Alice Y and Scott, Ian C and Gingras, Anne-Claude
Journal: Molecular & cellular proteomics : MCP (2021): 100128

In vivo discovery of RNA proximal proteins via proximity-dependent biotinylation.
Authors: Lin, Xianzhi and Fonseca, Marcos A S and Breunig, Joshua J and Corona, Rosario I and Lawrenson, Kate
Journal: RNA biology (2021): 1-15

Kinase-Catalyzed Biotinylation to Map Cell Signaling Pathways: Application to Epidermal Growth Factor Signaling.
Authors: Ramanayake-Mudiyanselage, Vindya and Embogama, D Maheeka and Pflum, Mary Kay H
Journal: Journal of proteome research (2021)

A proximity-dependent biotinylation map of a human cell.
Authors: Go, Christopher D and Knight, James D R and Rajasekharan, Archita and Rathod, Bhavisha and Hesketh, Geoffrey G and Abe, Kento T and Youn, Ji-Young and Samavarchi-Tehrani, Payman and Zhang, Hui and Zhu, Lucie Y and Popiel, Evelyn and Lambert, Jean-Philippe and Coyaud, Étienne and Cheung, Sally W T and Rajendran, Dushyandi and Wong, Cassandra J and Antonicka, Hana and Pelletier, Laurence and Palazzo, Alexander F and Shoubridge, Eric A and Raught, Brian and Gingras, Anne-Claude
Journal: Nature (2021): 120-124

Directly Quantifiable Biotinylation Using a Water-Soluble Isatoic Anhydride Platform.
Authors: Fessler, Adam B and Fowler, Anthony J and Ogle, Craig A
Journal: Bioconjugate chemistry (2021): 904-908

Rapid Enzyme-Mediated Biotinylation for Cell Surface Proteome Profiling.
Authors: Li, Yanan and Wang, Yan and Yao, Yating and Lyu, Jiawen and Qiao, Qinglong and Mao, Jiawei and Xu, Zhaochao and Ye, Mingliang
Journal: Analytical chemistry (2021): 4542-4551

Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells.
Authors: Zellner, Susanne and Nalbach, Karsten and Behrends, Christian
Journal: STAR protocols (2021): 100506

4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA.
Authors: Huang, Jinguo and Zhao, Ruiqi and Qin, Shanshan and Yang, Shixi and Li, Wei and Mo, Jing and Wang, Fang and Du, Yuhao and Weng, Xiaocheng and Zhou, Xiang
Journal: Chembiochem : a European journal of chemical biology (2021): 212-216