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ReadiCleave™ SSL biotin NHS ester

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Physical properties
Molecular weight504.64
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
ReadiCleave™ SSL biotin NHS ester is a cleavable biotinylating reagent which can efficiently introduce a biotin moiety to amine-containing biomolecules. This biotin compound contains a cleavable linker that can be cleaved using reducing agents such as DTT or TCEP to remove the biotin label when needed.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ SSL biotin NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM198.161 µL990.805 µL1.982 mL9.908 mL19.816 mL
5 mM39.632 µL198.161 µL396.322 µL1.982 mL3.963 mL
10 mM19.816 µL99.081 µL198.161 µL990.805 µL1.982 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ReadiCleave™ Cy5-SSL-NHS ester65167025000010.271, 0.420.020.03
ReadiCleave™ XFD532 SSL-NHS ester534553810000.6110.240.09
ReadiCleave™ FAM SSL-NHS ester491516730000.92-0.35



View all 48 references: Citation Explorer
In Situ Membrane Biotinylation Enables the Direct Labeling and Accurate Kinetic Analysis of Small Extracellular Vesicles in Circulation.
Authors: Yu, Zi-Li and Zhao, Yi and Miao, Fan and Wu, Min and Xia, Hou-Fu and Chen, Zhuo-Kun and Liu, Hai-Ming and Zhao, Yi-Fang and Chen, Gang
Journal: Analytical chemistry (2021): 10862-10870
Off-the-shelf proximity biotinylation for interaction proteomics.
Authors: Santos-Barriopedro, Irene and van Mierlo, Guido and Vermeulen, Michiel
Journal: Nature communications (2021): 5015
Coupling of Cell Surface Biotinylation and SILAC-Based Quantitative Proteomics Identified Myoferlin as a Potential Therapeutic Target for Nasopharyngeal Carcinoma Metastasis.
Authors: Li, Maoyu and Peng, Fang and Wang, Guoqiang and Liang, Xujun and Shao, Meiying and Chen, Zhuchu and Chen, Yongheng
Journal: Frontiers in cell and developmental biology (2021): 621810
Proximity-Dependent Biotinylation (BioID) of Integrin Interaction Partners.
Authors: Myllymäki, Satu-Marja and Liu, Xiaonan and Varjosalo, Markku and Manninen, Aki
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 57-69
Proximity-Dependent Biotinylation to Elucidate the Interactome of TNK2 Nonreceptor Tyrosine Kinase.
Authors: Tahir, Raiha and Madugundu, Anil K and Udainiya, Savita and Cutler, Jevon A and Renuse, Santosh and Wang, Li and Pearson, Nicole A and Mitchell, Christopher J and Mahajan, Nupam and Pandey, Akhilesh and Wu, Xinyan
Journal: Journal of proteome research (2021): 4566-4577
Thiol-Cleavable Biotin for Chemical and Enzymatic Biotinylation and Its Application to Mitochondrial TurboID Proteomics.
Authors: Li, Haorong and Frankenfield, Ashley M and Houston, Ryan and Sekine, Shiori and Hao, Ling
Journal: Journal of the American Society for Mass Spectrometry (2021): 2358-2365
Phenoxy Radical Reactivity of Nucleic Acids: Practical Implications for Biotinylation.
Authors: Wilbanks, Brandon and Garcia, Brian and Byrne, Shane and Dedon, Peter and Maher, L James
Journal: Chembiochem : a European journal of chemical biology (2021): 1400-1404
4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA.
Authors: Huang, Jinguo and Zhao, Ruiqi and Qin, Shanshan and Yang, Shixi and Li, Wei and Mo, Jing and Wang, Fang and Du, Yuhao and Weng, Xiaocheng and Zhou, Xiang
Journal: Chembiochem : a European journal of chemical biology (2021): 212-216
Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells.
Authors: Zellner, Susanne and Nalbach, Karsten and Behrends, Christian
Journal: STAR protocols (2021): 100506
Rapid Enzyme-Mediated Biotinylation for Cell Surface Proteome Profiling.
Authors: Li, Yanan and Wang, Yan and Yao, Yating and Lyu, Jiawen and Qiao, Qinglong and Mao, Jiawei and Xu, Zhaochao and Ye, Mingliang
Journal: Analytical chemistry (2021): 4542-4551