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ReadiCleave™ SSL biotin NHS ester

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Physical properties
Molecular weight504.64
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
ReadiCleave™ SSL biotin NHS ester is a cleavable biotinylating reagent which can efficiently introduce a biotin moiety to amine-containing biomolecules. This biotin compound contains a cleavable linker that can be cleaved using reducing agents such as DTT or TCEP to remove the biotin label when needed.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ SSL biotin NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM198.161 µL990.805 µL1.982 mL9.908 mL19.816 mL
5 mM39.632 µL198.161 µL396.322 µL1.982 mL3.963 mL
10 mM19.816 µL99.081 µL198.161 µL990.805 µL1.982 mL

Molarity calculator

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Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ReadiCleave™ Cy5-SSL-NHS ester65167025000010.271, 0.420.020.03
ReadiCleave™ XFD532 SSL-NHS ester534553810000.6110.240.09
ReadiCleave™ FAM SSL-NHS ester491516730000.92-0.35



View all 48 references: Citation Explorer
In Situ Membrane Biotinylation Enables the Direct Labeling and Accurate Kinetic Analysis of Small Extracellular Vesicles in Circulation.
Authors: Yu, Zi-Li and Zhao, Yi and Miao, Fan and Wu, Min and Xia, Hou-Fu and Chen, Zhuo-Kun and Liu, Hai-Ming and Zhao, Yi-Fang and Chen, Gang
Journal: Analytical chemistry (2021): 10862-10870
Tris(hydroxymethyl)aminomethane Compatibility with N-Hydroxysuccinimide Ester Chemistry: Biotinylation of Peptides and Proteins in TRIS Buffer.
Authors: Kratzer, Ulrich and Sommersdorf, Cornelia and Maier, Sandra and Wagner, Teresa R and Templin, Markus and Joos, Thomas O and Rothbauer, Ulrich and Zeck, Anne and Poetz, Oliver
Journal: Bioconjugate chemistry (2021)
A Toolbox for Efficient Proximity-Dependent Biotinylation in Zebrafish Embryos.
Authors: Rosenthal, Shimon M and Misra, Tvisha and Abdouni, Hala and Branon, Tess C and Ting, Alice Y and Scott, Ian C and Gingras, Anne-Claude
Journal: Molecular & cellular proteomics : MCP (2021): 100128
In vivo discovery of RNA proximal proteins via proximity-dependent biotinylation.
Authors: Lin, Xianzhi and Fonseca, Marcos A S and Breunig, Joshua J and Corona, Rosario I and Lawrenson, Kate
Journal: RNA biology (2021): 1-15
Kinase-Catalyzed Biotinylation to Map Cell Signaling Pathways: Application to Epidermal Growth Factor Signaling.
Authors: Ramanayake-Mudiyanselage, Vindya and Embogama, D Maheeka and Pflum, Mary Kay H
Journal: Journal of proteome research (2021)
A proximity-dependent biotinylation map of a human cell.
Authors: Go, Christopher D and Knight, James D R and Rajasekharan, Archita and Rathod, Bhavisha and Hesketh, Geoffrey G and Abe, Kento T and Youn, Ji-Young and Samavarchi-Tehrani, Payman and Zhang, Hui and Zhu, Lucie Y and Popiel, Evelyn and Lambert, Jean-Philippe and Coyaud, Étienne and Cheung, Sally W T and Rajendran, Dushyandi and Wong, Cassandra J and Antonicka, Hana and Pelletier, Laurence and Palazzo, Alexander F and Shoubridge, Eric A and Raught, Brian and Gingras, Anne-Claude
Journal: Nature (2021): 120-124
Directly Quantifiable Biotinylation Using a Water-Soluble Isatoic Anhydride Platform.
Authors: Fessler, Adam B and Fowler, Anthony J and Ogle, Craig A
Journal: Bioconjugate chemistry (2021): 904-908
Rapid Enzyme-Mediated Biotinylation for Cell Surface Proteome Profiling.
Authors: Li, Yanan and Wang, Yan and Yao, Yating and Lyu, Jiawen and Qiao, Qinglong and Mao, Jiawei and Xu, Zhaochao and Ye, Mingliang
Journal: Analytical chemistry (2021): 4542-4551
Autophagosome content profiling using proximity biotinylation proteomics coupled to protease digestion in mammalian cells.
Authors: Zellner, Susanne and Nalbach, Karsten and Behrends, Christian
Journal: STAR protocols (2021): 100506
4-Thiouridine-Enhanced Peroxidase-Generated Biotinylation of RNA.
Authors: Huang, Jinguo and Zhao, Ruiqi and Qin, Shanshan and Yang, Shixi and Li, Wei and Mo, Jing and Wang, Fang and Du, Yuhao and Weng, Xiaocheng and Zhou, Xiang
Journal: Chembiochem : a European journal of chemical biology (2021): 212-216