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ReadiCleave™ SSL biotin NHS ester
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Application notes
ReadiCleave™ SSL biotin NHS ester is a cleavable biotinylating reagent which can efficiently introduce a biotin moiety to amine-containing biomolecules. This biotin compound contains a cleavable linker that can be cleaved using reducing agents such as DTT or TCEP to remove the biotin label when needed.
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ReadiCleave™ Cy5-SSL-NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
| ReadiCleave™ XFD532 SSL-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| ReadiCleave™ FAM SSL-NHS ester | 491 | 516 | 73000 | 0.92 | - | 0.35 |
References
View all 48 references: Citation Explorer
In Situ Membrane Biotinylation Enables the Direct Labeling and Accurate Kinetic Analysis of Small Extracellular Vesicles in Circulation.
Authors: Yu, Zi-Li and Zhao, Yi and Miao, Fan and Wu, Min and Xia, Hou-Fu and Chen, Zhuo-Kun and Liu, Hai-Ming and Zhao, Yi-Fang and Chen, Gang
Journal: Analytical chemistry (2021): 10862-10870
Authors: Yu, Zi-Li and Zhao, Yi and Miao, Fan and Wu, Min and Xia, Hou-Fu and Chen, Zhuo-Kun and Liu, Hai-Ming and Zhao, Yi-Fang and Chen, Gang
Journal: Analytical chemistry (2021): 10862-10870
Off-the-shelf proximity biotinylation for interaction proteomics.
Authors: Santos-Barriopedro, Irene and van Mierlo, Guido and Vermeulen, Michiel
Journal: Nature communications (2021): 5015
Authors: Santos-Barriopedro, Irene and van Mierlo, Guido and Vermeulen, Michiel
Journal: Nature communications (2021): 5015
Coupling of Cell Surface Biotinylation and SILAC-Based Quantitative Proteomics Identified Myoferlin as a Potential Therapeutic Target for Nasopharyngeal Carcinoma Metastasis.
Authors: Li, Maoyu and Peng, Fang and Wang, Guoqiang and Liang, Xujun and Shao, Meiying and Chen, Zhuchu and Chen, Yongheng
Journal: Frontiers in cell and developmental biology (2021): 621810
Authors: Li, Maoyu and Peng, Fang and Wang, Guoqiang and Liang, Xujun and Shao, Meiying and Chen, Zhuchu and Chen, Yongheng
Journal: Frontiers in cell and developmental biology (2021): 621810
Proximity-Dependent Biotinylation (BioID) of Integrin Interaction Partners.
Authors: Myllymäki, Satu-Marja and Liu, Xiaonan and Varjosalo, Markku and Manninen, Aki
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 57-69
Authors: Myllymäki, Satu-Marja and Liu, Xiaonan and Varjosalo, Markku and Manninen, Aki
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 57-69
Proximity-Dependent Biotinylation to Elucidate the Interactome of TNK2 Nonreceptor Tyrosine Kinase.
Authors: Tahir, Raiha and Madugundu, Anil K and Udainiya, Savita and Cutler, Jevon A and Renuse, Santosh and Wang, Li and Pearson, Nicole A and Mitchell, Christopher J and Mahajan, Nupam and Pandey, Akhilesh and Wu, Xinyan
Journal: Journal of proteome research (2021): 4566-4577
Authors: Tahir, Raiha and Madugundu, Anil K and Udainiya, Savita and Cutler, Jevon A and Renuse, Santosh and Wang, Li and Pearson, Nicole A and Mitchell, Christopher J and Mahajan, Nupam and Pandey, Akhilesh and Wu, Xinyan
Journal: Journal of proteome research (2021): 4566-4577