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ReadiCleave™ XFD532 SSL-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ XFD 532 succinimidyl ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the Alexa Fluor 532 fluorophore from the target molecule. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.

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CatalogSize
Price
Quantity
70511 mg
Price
 
Physical properties

Molecular weight988.21
SolventDMSO
Spectral properties

Correction factor (260 nm)0.24
Correction factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)
81000
Excitation (nm)534
Emission (nm)553
Quantum yield
0.61
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Page updated on October 13, 2025