AAT Bioquest

ReadiCleave™ XFD532 SSL-NHS ester

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Physical properties
Molecular weight988.21
Spectral properties
Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)81000
Excitation (nm)534
Emission (nm)553
Quantum yield0.611
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ XFD 532 succinimidyl ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the Alexa Fluor 532 fluorophore from the target molecule. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ XFD532 SSL-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM101.193 µL505.965 µL1.012 mL5.06 mL10.119 mL
5 mM20.239 µL101.193 µL202.386 µL1.012 mL2.024 mL
10 mM10.119 µL50.597 µL101.193 µL505.965 µL1.012 mL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.24
Correction Factor (280 nm)0.09
Extinction coefficient (cm -1 M -1)81000
Excitation (nm)534
Emission (nm)553
Quantum yield0.611

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
ReadiCleave™ XFD532 AML-NHS ester534553810000.6110.09
ReadiCleave™ Cy5-SSL-NHS ester65167025000010.271, 0.420.03
ReadiCleave™ FAM SSL-NHS ester491516730000.920.35



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