ReadiCleave™ XFD532 SSL-NHS ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 988.21 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 534 |
Emission (nm) | 553 |
Quantum yield | 0.611 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | ![]() ![]() |
Molecular weight 988.21 | Correction Factor (260 nm) 0.24 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 81000 | Excitation (nm) 534 | Emission (nm) 553 | Quantum yield 0.611 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ XFD 532 succinimidyl ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the Alexa Fluor 532 fluorophore from the target molecule. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ XFD532 SSL-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 101.193 µL | 505.965 µL | 1.012 mL | 5.06 mL | 10.119 mL |
5 mM | 20.239 µL | 101.193 µL | 202.386 µL | 1.012 mL | 2.024 mL |
10 mM | 10.119 µL | 50.597 µL | 101.193 µL | 505.965 µL | 1.012 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 534 |
Emission (nm) | 553 |
Quantum yield | 0.611 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.09 |
ReadiCleave™ Cy5-SSL-NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 |
ReadiCleave™ FAM SSL-NHS ester | 491 | 516 | 73000 | 0.92 | 0.35 |
References
View all 17 references: Citation Explorer
Encapsulation of Plant Viral Particles in Calcite Crystals.
Authors: Al-Handawi, Marieh B and Commins, Patrick and Shukla, Sourabh and Didier, Pascal and Tanaka, Masahiko and Raj, Gijo and Veliz, Frank A and Pasricha, Renu and Steinmetz, Nicole F and Naumov, Panče
Journal: Advanced biosystems (2018): e1700176
Authors: Al-Handawi, Marieh B and Commins, Patrick and Shukla, Sourabh and Didier, Pascal and Tanaka, Masahiko and Raj, Gijo and Veliz, Frank A and Pasricha, Renu and Steinmetz, Nicole F and Naumov, Panče
Journal: Advanced biosystems (2018): e1700176
Multilayer Hydrogel Capsules of Interpenetrated Network for Encapsulation of Small Molecules.
Authors: Kozlovskaya, Veronika and Chen, Jun and Zavgorodnya, Oleksandra and Hasan, Mohammad B and Kharlampieva, Eugenia
Journal: Langmuir : the ACS journal of surfaces and colloids (2018): 11832-11842
Authors: Kozlovskaya, Veronika and Chen, Jun and Zavgorodnya, Oleksandra and Hasan, Mohammad B and Kharlampieva, Eugenia
Journal: Langmuir : the ACS journal of surfaces and colloids (2018): 11832-11842
Light emitting diode, photodiode-based fluorescence detection system for DNA analysis with microchip electrophoresis.
Authors: Hall, Gordon H and Glerum, D Moira and Backhouse, Christopher J
Journal: Electrophoresis (2016): 406-13
Authors: Hall, Gordon H and Glerum, D Moira and Backhouse, Christopher J
Journal: Electrophoresis (2016): 406-13
Assessing the efficacy of co-inoculation of wheat seedlings with the associative bacteria Paenibacillus polymyxa 1465 and Azospirillum brasilense Sp245.
Authors: Yegorenkova, Irina V and Tregubova, Kristina V and Burygin, Gennady L and Matora, Larisa Y and Ignatov, Vladimir V
Journal: Canadian journal of microbiology (2016): 279-85
Authors: Yegorenkova, Irina V and Tregubova, Kristina V and Burygin, Gennady L and Matora, Larisa Y and Ignatov, Vladimir V
Journal: Canadian journal of microbiology (2016): 279-85
Engineered drug-protein nanoparticle complexes for folate receptor targeting.
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Biochemical engineering journal (2014): 33-41
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Biochemical engineering journal (2014): 33-41
Fluorescence correlation spectroscopy with a doughnut-shaped excitation profile as a characterization tool in STED microscopy.
Authors: Tressler, Charmaine and Stolle, Michael and Fradin, Cécile
Journal: Optics express (2014): 31154-66
Authors: Tressler, Charmaine and Stolle, Michael and Fradin, Cécile
Journal: Optics express (2014): 31154-66
Calcium-regulated conformational change in the C-terminal end segment of troponin I and its binding to tropomyosin.
Authors: Zhang, Zhiling and Akhter, Shirin and Mottl, Steven and Jin, Jian-Ping
Journal: The FEBS journal (2011): 3348-59
Authors: Zhang, Zhiling and Akhter, Shirin and Mottl, Steven and Jin, Jian-Ping
Journal: The FEBS journal (2011): 3348-59
Protein nanocapsules containing doxorubicin as a pH-responsive delivery system.
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Small (Weinheim an der Bergstrasse, Germany) (2011): 1051-60
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Small (Weinheim an der Bergstrasse, Germany) (2011): 1051-60
Single-molecule immunosorbent assay as a tool for human immunodeficiency virus-1 antigen detection.
Authors: Li, Jiangwei and Xie, Wenjun and Fang, Ning and Yeung, Edward S
Journal: Analytical and bioanalytical chemistry (2009): 489-97
Authors: Li, Jiangwei and Xie, Wenjun and Fang, Ning and Yeung, Edward S
Journal: Analytical and bioanalytical chemistry (2009): 489-97
Real-time single-molecule kinetics of trypsin proteolysis.
Authors: Li, Jiangwei and Yeung, Edward S
Journal: Analytical chemistry (2008): 8509-13
Authors: Li, Jiangwei and Yeung, Edward S
Journal: Analytical chemistry (2008): 8509-13
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)