ReadiCleave™ XFD532 SSL-NHS ester
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ XFD 532 succinimidyl ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the Alexa Fluor 532 fluorophore from the target molecule. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.09 |
ReadiCleave™ Cy5-SSL-NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 |
ReadiCleave™ FAM SSL-NHS ester | 491 | 516 | 73000 | 0.92 | 0.35 |
References
View all 17 references: Citation Explorer
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Authors: Al-Handawi, Marieh B and Commins, Patrick and Shukla, Sourabh and Didier, Pascal and Tanaka, Masahiko and Raj, Gijo and Veliz, Frank A and Pasricha, Renu and Steinmetz, Nicole F and Naumov, Panče
Journal: Advanced biosystems (2018): e1700176
Authors: Al-Handawi, Marieh B and Commins, Patrick and Shukla, Sourabh and Didier, Pascal and Tanaka, Masahiko and Raj, Gijo and Veliz, Frank A and Pasricha, Renu and Steinmetz, Nicole F and Naumov, Panče
Journal: Advanced biosystems (2018): e1700176
Multilayer Hydrogel Capsules of Interpenetrated Network for Encapsulation of Small Molecules.
Authors: Kozlovskaya, Veronika and Chen, Jun and Zavgorodnya, Oleksandra and Hasan, Mohammad B and Kharlampieva, Eugenia
Journal: Langmuir : the ACS journal of surfaces and colloids (2018): 11832-11842
Authors: Kozlovskaya, Veronika and Chen, Jun and Zavgorodnya, Oleksandra and Hasan, Mohammad B and Kharlampieva, Eugenia
Journal: Langmuir : the ACS journal of surfaces and colloids (2018): 11832-11842
Light emitting diode, photodiode-based fluorescence detection system for DNA analysis with microchip electrophoresis.
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Journal: Electrophoresis (2016): 406-13
Authors: Hall, Gordon H and Glerum, D Moira and Backhouse, Christopher J
Journal: Electrophoresis (2016): 406-13
Assessing the efficacy of co-inoculation of wheat seedlings with the associative bacteria Paenibacillus polymyxa 1465 and Azospirillum brasilense Sp245.
Authors: Yegorenkova, Irina V and Tregubova, Kristina V and Burygin, Gennady L and Matora, Larisa Y and Ignatov, Vladimir V
Journal: Canadian journal of microbiology (2016): 279-85
Authors: Yegorenkova, Irina V and Tregubova, Kristina V and Burygin, Gennady L and Matora, Larisa Y and Ignatov, Vladimir V
Journal: Canadian journal of microbiology (2016): 279-85
Engineered drug-protein nanoparticle complexes for folate receptor targeting.
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Biochemical engineering journal (2014): 33-41
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Biochemical engineering journal (2014): 33-41
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