ReadiCleave™ FITC AML-NHS ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 966.98 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
Molecular weight 966.98 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML FITC contains an azidomethyl linker that can be cleaved with TCEP to remove the fluorescein fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ FITC AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 103.415 µL | 517.074 µL | 1.034 mL | 5.171 mL | 10.341 mL |
5 mM | 20.683 µL | 103.415 µL | 206.83 µL | 1.034 mL | 2.068 mL |
10 mM | 10.341 µL | 51.707 µL | 103.415 µL | 517.074 µL | 1.034 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ XFD488 AML-NHS ester | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
ReadiCleave™ XFD546 AML-NHS ester | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
View all 50 references: Citation Explorer
Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Biomedical and Pharmacological Uses of Fluorescein Isothiocyanate Chitosan-Based Nanocarriers.
Authors: Caprifico, Anna E and Polycarpou, Elena and Foot, Peter J S and Calabrese, Gianpiero
Journal: Macromolecular bioscience (2021): e2000312
Authors: Caprifico, Anna E and Polycarpou, Elena and Foot, Peter J S and Calabrese, Gianpiero
Journal: Macromolecular bioscience (2021): e2000312
Research Note: Modified serum fluorescein isothiocyanate dextran (FITC-d) assay procedure to determine intestinal permeability in poultry fed diets high in natural or synthetic pigments.
Authors: Vuong, Christine N and Mullenix, Garrett J and Kidd, Michael T and Bottje, Walter G and Hargis, Billy M and Tellez-Isaias, Guillermo
Journal: Poultry science (2021): 101138
Authors: Vuong, Christine N and Mullenix, Garrett J and Kidd, Michael T and Bottje, Walter G and Hargis, Billy M and Tellez-Isaias, Guillermo
Journal: Poultry science (2021): 101138
Influence of Liver Intoxication by Carbon Tetrachloride or D-Galactosamine on Absorption of Fluorescein Isothiocyanate-Dextran-10 and Other Marker Compounds with Different Molecular Weights from the Rat Liver Surface.
Authors: Miyamoto, Hirotaka and Tsuda, Kayoko and Honda, Tominori and Tokunaga, Ayako and Fumoto, Shintaro and Nishida, Koyo
Journal: Biological & pharmaceutical bulletin (2020): 319-324
Authors: Miyamoto, Hirotaka and Tsuda, Kayoko and Honda, Tominori and Tokunaga, Ayako and Fumoto, Shintaro and Nishida, Koyo
Journal: Biological & pharmaceutical bulletin (2020): 319-324
Development of fluorescein isothiocyanate conjugated gellan gum for application of bioimaging for biomedical application.
Authors: Cho, Hun Hwi and Choi, Joo Hee and Been, Su Young and Kim, Namyeong and Choi, Jeong Min and Kim, Wooyoup and Kim, David and Jung, Jun Jae and Song, Jeong Eun and Khang, Gilson
Journal: International journal of biological macromolecules (2020): 2804-2812
Authors: Cho, Hun Hwi and Choi, Joo Hee and Been, Su Young and Kim, Namyeong and Choi, Jeong Min and Kim, Wooyoup and Kim, David and Jung, Jun Jae and Song, Jeong Eun and Khang, Gilson
Journal: International journal of biological macromolecules (2020): 2804-2812
Fluorescein isothiocyanate and blue light irradiation alter cell-adhesiveness of cross-linked albumin films for cell patterning.
Authors: Tachibana, Akira and Iida, Atsuko and Tanabe, Toshizumi
Journal: Bioscience, biotechnology, and biochemistry (2020): 800-803
Authors: Tachibana, Akira and Iida, Atsuko and Tanabe, Toshizumi
Journal: Bioscience, biotechnology, and biochemistry (2020): 800-803
Technical note: fluorescein as an indicator of enteric mucosal barrier function in preruminant lambs.
Authors: Duff, Audrey F and Bielke, Lisa R and Relling, Alejandro E
Journal: Journal of animal science (2020)
Authors: Duff, Audrey F and Bielke, Lisa R and Relling, Alejandro E
Journal: Journal of animal science (2020)
Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa.
Authors: Rajabi-Toustani, Reza and Akter, Quzi Sharmin and Almadaly, Essam A and Hoshino, Yoichiro and Adachi, Hiromichi and Mukoujima, Koushi and Murase, Tetsuma
Journal: The Journal of veterinary medical science (2019): 694-702
Authors: Rajabi-Toustani, Reza and Akter, Quzi Sharmin and Almadaly, Essam A and Hoshino, Yoichiro and Adachi, Hiromichi and Mukoujima, Koushi and Murase, Tetsuma
Journal: The Journal of veterinary medical science (2019): 694-702
Molecular characterization of the β-lactoglobulin conjugated with fluorescein isothiocyanate: Binding sites and structure changes as function of pH.
Authors: Zhang, Xiaoning and Hemar, Yacine and Lv, Lei and Zhao, Tao and Yang, Yuhong and Han, Zhonghui and Li, Meifeng and He, Jinxing
Journal: International journal of biological macromolecules (2019): 377-383
Authors: Zhang, Xiaoning and Hemar, Yacine and Lv, Lei and Zhao, Tao and Yang, Yuhong and Han, Zhonghui and Li, Meifeng and He, Jinxing
Journal: International journal of biological macromolecules (2019): 377-383
Competitive and noncompetitive immunoassays for the detection of benzothiostrobin using magnetic nanoparticles and fluorescein isothiocyanate-labeled peptides.
Authors: Chen, He and Yang, Qian and Ding, Yuan and Vasylieva, Natalia and Bever, Candace S and Hua, Xiude and Wang, Minghua and Hammock, Bruce D
Journal: Analytical and bioanalytical chemistry (2019): 527-535
Authors: Chen, He and Yang, Qian and Ding, Yuan and Vasylieva, Natalia and Bever, Candace S and Hua, Xiude and Wang, Minghua and Hammock, Bruce D
Journal: Analytical and bioanalytical chemistry (2019): 527-535