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AAT Bioquest

ReadiCleave™ FITC AML-NHS ester

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Physical properties
Molecular weight966.98
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
966.98
Absorbance (nm)
487
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.35
Extinction coefficient (cm -1 M -1)
800001
Excitation (nm)
498
Emission (nm)
517
Quantum yield
0.79001, 0.952
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML FITC contains an azidomethyl linker that can be cleaved with TCEP to remove the fluorescein fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ FITC AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM103.415 µL517.074 µL1.034 mL5.171 mL10.341 mL
5 mM20.683 µL103.415 µL206.83 µL1.034 mL2.068 mL
10 mM10.341 µL51.707 µL103.415 µL517.074 µL1.034 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ReadiCleave™ AML Cy5 NHS ester65167025000010.271, 0.420.020.03
ReadiCleave™ XFD532 AML-NHS ester534553810000.6110.240.09
ReadiCleave™ XFD488 AML-NHS ester499520730000.9210.30.11
ReadiCleave™ XFD546 AML-NHS ester5615721120000.7910.210.12
ReadiCleave™ XFD647 AML-NHS ester6506712390000.3310.000.03

Images


References


View all 50 references: Citation Explorer
Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Biomedical and Pharmacological Uses of Fluorescein Isothiocyanate Chitosan-Based Nanocarriers.
Authors: Caprifico, Anna E and Polycarpou, Elena and Foot, Peter J S and Calabrese, Gianpiero
Journal: Macromolecular bioscience (2021): e2000312
Research Note: Modified serum fluorescein isothiocyanate dextran (FITC-d) assay procedure to determine intestinal permeability in poultry fed diets high in natural or synthetic pigments.
Authors: Vuong, Christine N and Mullenix, Garrett J and Kidd, Michael T and Bottje, Walter G and Hargis, Billy M and Tellez-Isaias, Guillermo
Journal: Poultry science (2021): 101138
Influence of Liver Intoxication by Carbon Tetrachloride or D-Galactosamine on Absorption of Fluorescein Isothiocyanate-Dextran-10 and Other Marker Compounds with Different Molecular Weights from the Rat Liver Surface.
Authors: Miyamoto, Hirotaka and Tsuda, Kayoko and Honda, Tominori and Tokunaga, Ayako and Fumoto, Shintaro and Nishida, Koyo
Journal: Biological & pharmaceutical bulletin (2020): 319-324
Development of fluorescein isothiocyanate conjugated gellan gum for application of bioimaging for biomedical application.
Authors: Cho, Hun Hwi and Choi, Joo Hee and Been, Su Young and Kim, Namyeong and Choi, Jeong Min and Kim, Wooyoup and Kim, David and Jung, Jun Jae and Song, Jeong Eun and Khang, Gilson
Journal: International journal of biological macromolecules (2020): 2804-2812
Fluorescein isothiocyanate and blue light irradiation alter cell-adhesiveness of cross-linked albumin films for cell patterning.
Authors: Tachibana, Akira and Iida, Atsuko and Tanabe, Toshizumi
Journal: Bioscience, biotechnology, and biochemistry (2020): 800-803
Technical note: fluorescein as an indicator of enteric mucosal barrier function in preruminant lambs.
Authors: Duff, Audrey F and Bielke, Lisa R and Relling, Alejandro E
Journal: Journal of animal science (2020)
Methodological improvement of fluorescein isothiocyanate peanut agglutinin (FITC-PNA) acrosomal integrity staining for frozen-thawed Japanese Black bull spermatozoa.
Authors: Rajabi-Toustani, Reza and Akter, Quzi Sharmin and Almadaly, Essam A and Hoshino, Yoichiro and Adachi, Hiromichi and Mukoujima, Koushi and Murase, Tetsuma
Journal: The Journal of veterinary medical science (2019): 694-702
Molecular characterization of the β-lactoglobulin conjugated with fluorescein isothiocyanate: Binding sites and structure changes as function of pH.
Authors: Zhang, Xiaoning and Hemar, Yacine and Lv, Lei and Zhao, Tao and Yang, Yuhong and Han, Zhonghui and Li, Meifeng and He, Jinxing
Journal: International journal of biological macromolecules (2019): 377-383
Competitive and noncompetitive immunoassays for the detection of benzothiostrobin using magnetic nanoparticles and fluorescein isothiocyanate-labeled peptides.
Authors: Chen, He and Yang, Qian and Ding, Yuan and Vasylieva, Natalia and Bever, Candace S and Hua, Xiude and Wang, Minghua and Hammock, Bruce D
Journal: Analytical and bioanalytical chemistry (2019): 527-535