ReadiCleave™ iFluor 488 AML-NHS ester
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1340.49 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
Molecular weight 1340.49 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML iFluor® 488 contains an azidomethyl linker that can be cleaved with TCEP to remove the iFluor® 488 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. iFluor® 488 is a superior replacement to Alexa Fluor® 488. iFluor® 488 and Alexa Fluor® 488 have very similar spectral properties.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ iFluor 488 AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 74.6 µL | 372.998 µL | 745.996 µL | 3.73 mL | 7.46 mL |
5 mM | 14.92 µL | 74.6 µL | 149.199 µL | 745.996 µL | 1.492 mL |
10 mM | 7.46 µL | 37.3 µL | 74.6 µL | 372.998 µL | 745.996 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ iFluor 700 AML-NHS ester | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
ReadiCleave™ iFluor 546 AML-NHS ester | 541 | 557 | 1000001 | 0.671 | 0.25 | 0.15 |
ReadiCleave™ iFluor 594 AML-NHS ester | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
ReadiCleave™ iFluor 488 SSL-NHS ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
References
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Authors: Zubova, Svetlana V and Grachev, Sergey V and Prokhorenko, Isabella R
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