ReadiCleave™ XFD546 AML-NHS ester
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1553.00 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 112000 |
Excitation (nm) | 561 |
Emission (nm) | 572 |
Quantum yield | 0.791 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Direct upgrades
ReadiCleave™ iFluor 546 AML-NHS ester |
Related products
Overview | SDSProtocol |
Molecular weight 1553.00 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.12 | Extinction coefficient (cm -1 M -1) 112000 | Excitation (nm) 561 | Emission (nm) 572 | Quantum yield 0.791 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML XFD546 contains an azidomethyl linker that can be cleaved with TCEP to remove the Alexa Fluor® 546 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. XFD546 is the same fluorophore to Alexa Fluor® 546 (Alexa Fluor® is the trademark of ThermoFisher).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ XFD546 AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 64.392 µL | 321.958 µL | 643.915 µL | 3.22 mL | 6.439 mL |
5 mM | 12.878 µL | 64.392 µL | 128.783 µL | 643.915 µL | 1.288 mL |
10 mM | 6.439 µL | 32.196 µL | 64.392 µL | 321.958 µL | 643.915 µL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.12 |
Extinction coefficient (cm -1 M -1) | 112000 |
Excitation (nm) | 561 |
Emission (nm) | 572 |
Quantum yield | 0.791 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ FITC AML-NHS ester | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
ReadiCleave™ XFD488 AML-NHS ester | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
View all 40 references: Citation Explorer
Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Authors: Ahishali, Bulent and Kaya, Mehmet
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 87-103
Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series*.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021)
Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546
Authors: Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546
Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568.
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Authors: Gajdos, Tamás and Hopp, Béla and Erdélyi, Miklós
Journal: Journal of fluorescence (2020): 437-443
Photo-isomerization of the Cyanine Dye Alexa-Fluor 647 (AF-647) in the Context of dSTORM Super-Resolution Microscopy.
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998
Authors: Karlsson, Joshua K G and Laude, Alex and Hall, Michael J and Harriman, Anthony
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 14983-14998
Observing the Reversible Single Molecule Electrochemistry of Alexa Fluor 647 Dyes by Total Internal Reflection Fluorescence Microscopy.
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498
Authors: Fan, Sanjun and Webb, James E A and Yang, Ying and Nieves, Daniel J and Gonçales, Vinicius R and Tran, Jason and Hilzenrat, Geva and Kahram, Mohaddeseh and Tilley, Richard D and Gaus, Katharina and Gooding, J Justin
Journal: Angewandte Chemie (International ed. in English) (2019): 14495-14498
Temporal Distribution Patterns of Alexa Fluor 647-Conjugated CeNPs in the Mouse Retina After a Single Intravitreal Injection.
Authors: Wong, Lily L and Barkam, Swetha and Seal, Sudipta and McGinnis, James F
Journal: Advances in experimental medicine and biology (2019): 125-130
Authors: Wong, Lily L and Barkam, Swetha and Seal, Sudipta and McGinnis, James F
Journal: Advances in experimental medicine and biology (2019): 125-130
Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line.
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29
Authors: Ostad, Seyed Nasser and Babaei, Sepideh and Bayat, Ali Ahmad and Mahmoudian, Jafar
Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (2019): 25-29