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Acridine orange

Chemical structure for Acridine orange
Chemical structure for Acridine orange
Ordering information
Price ()
Catalog Number17502
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight301.82
Spectral properties
Absorbance (nm)487
Extinction coefficient (cm -1 M -1)27000
Excitation (nm)490
Emission (nm)520
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). The dye is often used in epifluorescence microscopy.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Acridine orange to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM331.323 µL1.657 mL3.313 mL16.566 mL33.132 mL
5 mM66.265 µL331.323 µL662.647 µL3.313 mL6.626 mL
10 mM33.132 µL165.662 µL331.323 µL1.657 mL3.313 mL

Molarity calculator

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Spectral properties

Absorbance (nm)487
Extinction coefficient (cm -1 M -1)27000
Excitation (nm)490
Emission (nm)520

Product family

NameExcitation (nm)Emission (nm)
Thiazole orange *CAS 107091-89-4*514533
Thiazole orange *10 mM in DMSO* 514533
CytoTell™ Orange541560
LysoBrite™ Orange543565


View all 56 references: Citation Explorer
Spectral studies of N-nonyl acridine orange in anionic, cationic and neutral surfactants
Authors: Wiosetek-Reske AM, Wysocki S.
Journal: Spectrochim Acta A Mol Biomol Spectrosc (2006): 1118
Evaluation of the gram stain-acridine orange leukocyte cytospin test for diagnosis of catheter-related bloodstream infection in children on long-term parenteral nutrition
Authors: Ferroni A, Moumile K, Pasquier A, Berche P, Colomb V.
Journal: Eur J Clin Microbiol Infect Dis (2006): 199
The interaction of acridine orange with anionic surfactant and its application to the determination of protein
Authors: Shi Y, Zheng WW, Wu XX, Zhou DH.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 1653
Acridine orange accumulation in acid organelles of normal and vacuolated frog skeletal muscle fibres
Authors: Krolenko SA, Adamyan SY, Belyaeva TN, Mozhenok TP.
Journal: Cell Biol Int (2006): 933
Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306
Postoperative changes of sperm chromatin heterogeneity, using acridine orange staining, in varicocele patients
Authors: Fuse H, Akashi T, Mizuno I, Nozaki T, Watanabe A.
Journal: Arch Androl (2006): 223
Study on the formation and depolymerization of acridine orange dimer in acridine orange-sodium dodecyl benzene sulfonate-protein system
Authors: Wang F, Yang J, Wu X, Wang X, Feng L, Jia Z, Guo C.
Journal: J Colloid Interface Sci (2006): 757
Acridine orange used for photodynamic therapy accumulates in malignant musculoskeletal tumors depending on pH gradient
Authors: Matsubara T, Kusuzaki K, Matsumine A, Shintani K, Satonaka H, Uchida A.
Journal: Anticancer Res (2006): 187
Mutagenic effect of acridine orange on the expression of penicillin G acylase and beta-lactamase in Escherichia coli
Authors: Arshad R, Farooq S, Iqbal N, Ali SS.
Journal: Lett Appl Microbiol (2006): 94
Detection of catheter-related bloodstream infections by the Gram stain-acridine orange leukocyte cytospin test in hematopoietic stem cell transplant recipients
Authors: Abdelkefi A, Achour W, Torjman L, Ben Othman T, Ladeb S, Lakhal A, Allouche H, Ben Hassen A, Ben Abdeladhim A.
Journal: Bone Marrow Transplant (2006): 595