Calculator
Ala-AMC
Safety data sheetFAQ
Application notes
Ala-AMC is a sensitive fluorogenic protease substrate. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. It has been used for monitoring the protease activity of alanyl aminopeptidase and trypsin.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Ala-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 277.562 µL | 1.388 mL | 2.776 mL | 13.878 mL | 27.756 mL |
| 5 mM | 55.512 µL | 277.562 µL | 555.124 µL | 2.776 mL | 5.551 mL |
| 10 mM | 27.756 µL | 138.781 µL | 277.562 µL | 1.388 mL | 2.776 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Pro-AMC | 341 | 441 |
| Leu-AMC [L-Leucine-7-amido-4-methylcoumarin] *CAS 62480-44-8* | 341 | 441 |
| Ala-R110 | 500 | 522 |
References
View all 79 references: Citation Explorer
A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195
Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616
A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1
A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121
Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454