AAT Bioquest

Aminopropargyl dUTP [5-Propargylamino-2'-deoxyuridine-5'-triphosphate]

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Physical properties
Molecular weight544.93
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
The amine-modified deoxyuridine 5'-triphosphates (such as 5-propargylamino-2'-deoxyuridine-5'-triphosphate) can be used to produce amine-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. Aminopropargyl dUTP can be readily incorporated into DNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminopropargyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled nucleotides that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Aminopropargyl dUTP [5-Propargylamino-2'-deoxyuridine-5'-triphosphate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM183.51 µL917.549 µL1.835 mL9.175 mL18.351 mL
5 mM36.702 µL183.51 µL367.02 µL1.835 mL3.67 mL
10 mM18.351 µL91.755 µL183.51 µL917.549 µL1.835 mL

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View all 3 references: Citation Explorer
Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919
Fluorescent labelling of cRNA for microarray applications
Authors: t Hoen PA, de Kort F, van Ommen GJ, den Dunnen JT.
Journal: Nucleic Acids Res (2003): e20
Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA
Authors: Chen CB, Gorin MB, Sigman DS.
Journal: Proc Natl Acad Sci U S A (1993): 4206