ATTO 514 NHS ester

ATTO 514 NHS ester is an amine‑reactive, green fluorescent dye with an excitation/emission of 510/531 nm that is used for FACS and FISH applications.

Rapid Amine Reactivity: Forms stable amide bonds with peptides, amine‑modified oligonucleotides, and surface aminosilanes
Superior Brightness & Photostability: Delivers sustained fluorescence for high‑resolution techniques
Moderate Hydrophilicity: Prevents unwanted aggregation in aqueous environments

Chemical structure for ATTO 514 NHS ester.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2863	1 mg	Price

Overview

ATTO 514 NHS ester is manufactured by AAT Bioquest for research and development use. ATTO 514, a hydrophilic rhodamine-based fluorophore, is known for its excellent water solubility, strong absorption, high fluorescence quantum yield, and thermal and photo-stability. It is well-suited for single-molecule detection and high-resolution microscopy techniques like PALM, dSTORM, and STED. Additionally, it finds applications in flow cytometry (FACS), fluorescence in-situ hybridization (FISH), and other scientific methodologies. ATTO 514 exhibits optimal fluorescence efficiency when excited within the 510-535 nm range, making it a match for the 514 nm line of an Argon-Ion laser. ATTO 514 NHS ester is a popular tool used to label the primary amines of peptides, and amino-modified oligonucleotides. For labeling antibodies and other proteins, we highly recommend our iFluor® dyes that are highly water soluble and are optimized for labeling proteins.

References

View all 50 references: Citation Explorer

Improved enzymatic labeling of fluorescent in situ hybridization probes applied to the visualization of retained introns in cells.

Authors:

Xiao, Wen and Yeom, Kyu-Hyeon and Lin, Chia-Ho and Black, Douglas L

Journal:

RNA (New York, N.Y.) (2023)

Measuring Photophysical Transition Rates with Fluorescence Correlation Spectroscopy and Antibunching.

Authors:

Sakhapov, Damir and Gregor, Ingo and Karedla, Narain and Enderlein, Jörg

Journal:

The journal of physical chemistry letters (2022): 4823-4830

Combining Fluorescence Fluctuations and Photobleaching to Quantify Surface Density.

Authors:

Sefkow-Werner, Julius and Migliorini, Elisa and Picart, Catherine and Wahyuni, Dwiria and Wang, Irène and Delon, Antoine

Journal:

Analytical chemistry (2022): 6521-6528

A Model of F-actin Organization in Granuloreticulopodia in Foraminifera: Morphogenetic and Evolutionary Implications from Novel Fluorescent and Polarised Light Observations.

Authors:

Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max

Journal:

Protist (2022): 125886

Fractional CO2 laser ablation leads to enhanced permeation of a fluorescent dye in healthy and mycotic nails-An imaging investigation of laser-tissue effects and their impact on ungual drug delivery.

Authors:

Ortner, Vinzent Kevin and Nguyen, Nhi and Brewer, Jonathan R and Solovyeva, Vita and Haedersdal, Merete and Philipsen, Peter Alshede

Journal:

Lasers in surgery and medicine (2022): 861-874

Physical properties

Molecular weight	951.91
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.08
Extinction coefficient (cm ^-1 M ^-1)	115,000
Excitation (nm)	510
Emission (nm)	531
Quantum yield	0.85

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 17, 2025