Cal-520L™ maleimide

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cal-520L has been proven to be one of the best green fluorescent calcium indicators as a low affinity calcium indicator. Conceivably this thiol-reactive Cal-520L maleimide could be coupled to an IgG or other carrier molecules to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20611	100 ug	Price

References

View all 50 references: Citation Explorer

Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision.

Authors:

Makowka, Philipp and Bruegmann, Tobias and Dusend, Vanessa and Malan, Daniela and Beiert, Thomas and Hesse, Michael and Fleischmann, Bernd K and Sasse, Philipp

Journal:

Nature communications (2019): 1281

Polymer microchamber arrays for geometry-controlled drug release: a functional study in human cells of neuronal phenotype.

Authors:

Kopach, Olga and Zheng, Kayiu and Sindeeva, Olga A and Gai, Meiyu and Sukhorukov, Gleb B and Rusakov, Dmitri A

Journal:

Biomaterials science (2019): 2358-2371

Red-Shifted Aequorin Variants Incorporating Non-Canonical Amino Acids: Applications in In Vivo Imaging.

Authors:

Grinstead, Kristen M and Rowe, Laura and Ensor, Charles M and Joel, Smita and Daftarian, Pirouz and Dikici, Emre and Zingg, Jean-Marc and Daunert, Sylvia

Journal:

PloS one (2016): e0158579

Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein.

Authors:

Yang, Sung-Tae and Lim, Sung In and Kiessling, Volker and Kwon, Inchan and Tamm, Lukas K

Journal:

Scientific reports (2016): 32866

Mapping Calcium-Sensitive Regions in the Neuronal Calcium Sensor GCAP2 by Site-Specific Fluorescence Labeling.

Authors:

Sulmann, Stefan and Wallisch, Melanie and Scholten, Alexander and Christoffers, Jens and Koch, Karl-Wilhelm

Journal:

Biochemistry (2016): 2567-77

Physical properties

Molecular weight	991.78
Solvent	DMSO

Spectral properties

Excitation (nm)	492
Emission (nm)	515
Quantum yield	0.75 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 26, 2025