Cal-520L™ maleimide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 991.78 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Calcium Indicators
Molecular weight 991.78 | Excitation (nm) 493 | Emission (nm) 515 | Quantum yield 0.751 |
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cal-520L has been proven to be one of the best green fluorescent calcium indicators as a low affinity calcium indicator. Conceivably this thiol-reactive Cal-520L maleimide could be coupled to an IgG or other carrier molecules to prepare a calcium-sensitive bioconjugate for monitoring calcium change spatially for a specific target.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cal-520L™ maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 100.829 µL | 504.144 µL | 1.008 mL | 5.041 mL | 10.083 mL |
5 mM | 20.166 µL | 100.829 µL | 201.658 µL | 1.008 mL | 2.017 mL |
10 mM | 10.083 µL | 50.414 µL | 100.829 µL | 504.144 µL | 1.008 mL |
Molarity calculator
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Spectrum
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Spectral properties
Excitation (nm) | 493 |
Emission (nm) | 515 |
Quantum yield | 0.751 |
References
View all 50 references: Citation Explorer
Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision.
Authors: Makowka, Philipp and Bruegmann, Tobias and Dusend, Vanessa and Malan, Daniela and Beiert, Thomas and Hesse, Michael and Fleischmann, Bernd K and Sasse, Philipp
Journal: Nature communications (2019): 1281
Authors: Makowka, Philipp and Bruegmann, Tobias and Dusend, Vanessa and Malan, Daniela and Beiert, Thomas and Hesse, Michael and Fleischmann, Bernd K and Sasse, Philipp
Journal: Nature communications (2019): 1281
Polymer microchamber arrays for geometry-controlled drug release: a functional study in human cells of neuronal phenotype.
Authors: Kopach, Olga and Zheng, Kayiu and Sindeeva, Olga A and Gai, Meiyu and Sukhorukov, Gleb B and Rusakov, Dmitri A
Journal: Biomaterials science (2019): 2358-2371
Authors: Kopach, Olga and Zheng, Kayiu and Sindeeva, Olga A and Gai, Meiyu and Sukhorukov, Gleb B and Rusakov, Dmitri A
Journal: Biomaterials science (2019): 2358-2371
Red-Shifted Aequorin Variants Incorporating Non-Canonical Amino Acids: Applications in In Vivo Imaging.
Authors: Grinstead, Kristen M and Rowe, Laura and Ensor, Charles M and Joel, Smita and Daftarian, Pirouz and Dikici, Emre and Zingg, Jean-Marc and Daunert, Sylvia
Journal: PloS one (2016): e0158579
Authors: Grinstead, Kristen M and Rowe, Laura and Ensor, Charles M and Joel, Smita and Daftarian, Pirouz and Dikici, Emre and Zingg, Jean-Marc and Daunert, Sylvia
Journal: PloS one (2016): e0158579
Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein.
Authors: Yang, Sung-Tae and Lim, Sung In and Kiessling, Volker and Kwon, Inchan and Tamm, Lukas K
Journal: Scientific reports (2016): 32866
Authors: Yang, Sung-Tae and Lim, Sung In and Kiessling, Volker and Kwon, Inchan and Tamm, Lukas K
Journal: Scientific reports (2016): 32866
Mapping Calcium-Sensitive Regions in the Neuronal Calcium Sensor GCAP2 by Site-Specific Fluorescence Labeling.
Authors: Sulmann, Stefan and Wallisch, Melanie and Scholten, Alexander and Christoffers, Jens and Koch, Karl-Wilhelm
Journal: Biochemistry (2016): 2567-77
Authors: Sulmann, Stefan and Wallisch, Melanie and Scholten, Alexander and Christoffers, Jens and Koch, Karl-Wilhelm
Journal: Biochemistry (2016): 2567-77
A Water Framework Directive-compatible metric for assessing acidification in UK and Irish rivers using diatoms.
Authors: Juggins, Steve and Kelly, Martyn and Allott, Tim and Kelly-Quinn, Mary and Monteith, Don
Journal: The Science of the total environment (2016): 671-678
Authors: Juggins, Steve and Kelly, Martyn and Allott, Tim and Kelly-Quinn, Mary and Monteith, Don
Journal: The Science of the total environment (2016): 671-678
Is FEV(1) an indicator of low bone mineral density in adults? The Fifth Korea National Health and Nutrition Examination Survey.
Authors: Lee, Kayoung
Journal: Journal of bone and mineral metabolism (2015): 335-41
Authors: Lee, Kayoung
Journal: Journal of bone and mineral metabolism (2015): 335-41
Excited-state structural dynamics of a dual-emission calmodulin-green fluorescent protein sensor for calcium ion imaging.
Authors: Oscar, Breland G and Liu, Weimin and Zhao, Yongxin and Tang, Longteng and Wang, Yanli and Campbell, Robert E and Fang, Chong
Journal: Proceedings of the National Academy of Sciences of the United States of America (2014): 10191-6
Authors: Oscar, Breland G and Liu, Weimin and Zhao, Yongxin and Tang, Longteng and Wang, Yanli and Campbell, Robert E and Fang, Chong
Journal: Proceedings of the National Academy of Sciences of the United States of America (2014): 10191-6
Optogenetic control of ROS production.
Authors: Wojtovich, Andrew P and Foster, Thomas H
Journal: Redox biology (2014): 368-76
Authors: Wojtovich, Andrew P and Foster, Thomas H
Journal: Redox biology (2014): 368-76
Copper-64 radiolabelling of the C2A domain of synaptotagmin I using a functionalised bis(thiosemicarbazone): A pre- and post-labelling comparison.
Authors: Hueting, Rebekka and Tavaré, Richard and Dilworth, Jonathan R and Mullen, Gregory E
Journal: Journal of inorganic biochemistry (2013): 108-11
Authors: Hueting, Rebekka and Tavaré, Richard and Dilworth, Jonathan R and Mullen, Gregory E
Journal: Journal of inorganic biochemistry (2013): 108-11
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Does EDTA inactivate proteinase K?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Does EDTA inactivate proteinase K?