Cell Meter™ Mitochondrial Autophagy Imaging Kit Red Fluorescence

The fluorescence images of HeLa cells co-stained with Mitophagy Red™ and Hoechst 33342 (Cat#17535) in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) addition of CCCP (10 uM) for 1 minute. The cells were imaged using a fluorescence microscope with a Cy3/TRITC filter.

The fluorescence images of HeLa cells co-stained with Mitophagy Red™ and MitoLite™ Green FM (Cat#22695) in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) addition of CCCP (10 uM) for 1 minute. The cells were imaged using a fluorescence microscope with a Cy3/TRITC filter for Mitophagy Red™ and FITC filter for MitoLite™ Green FM and overlapped with each other.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Overview SDS Protocol

Mitochondrial autophagy (also called ”mitophagy”) appears to be involved in Alzheimer and Parkinson diseases, mainly induced by the accumulation of depolarized mitochondria. Mitophagy serves as an elimination system that removes dysfunctional mitochondria caused by oxidative stress and DNA damage, causing sequestration into auto phagosome, followed by fusion to lysosome and is degraded. Cell Meter™ Mitochondrial Autophagy Mitophagy Iimaging Kit uses Mitophagy Red™ as the mitophagy probe, which enables the very rapid and uniform staining of mitochondria across a wide variety of mammalian cell types and translocate to lysosome upon induction of mitophagy. The Cell Meter™ Mitochondrial Autophagy Imaging Kit provides an excellent tool to be used as an indicator of mitophagy in suspended or attached live cells. The staining pattern of Mitophagy Red™in live cells is stable enough that it provides enough time for studying most live cell dynamic. The assay conditions are compatible with cell culture medium. The excitation/emission of the Mitophagy Red™ probe fits well the widely available Cy3/TRITC filter set and can easily be combined with GFP expressed cell lines if desired.

Platform

Fluorescence microscope

Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy3/TRITC filter set

Components

Images

Figure 1. The fluorescence images of HeLa cells co-stained with Mitophagy Red™ and Hoechst 33342 (Cat#17535) in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) addition of CCCP (10 uM) for 1 minute. The cells were imaged using a fluorescence microscope with a Cy3/TRITC filter.

Figure 2. The fluorescence images of HeLa cells co-stained with Mitophagy Red™ and MitoLite™ Green FM (Cat#22695) in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) addition of CCCP (10 uM) for 1 minute. The cells were imaged using a fluorescence microscope with a Cy3/TRITC filter for Mitophagy Red™ and FITC filter for MitoLite™ Green FM and overlapped with each other.