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Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit
Optimized for Microplate Reader
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the loss of the mitochondrial membrane potential. The collapse of mitochondrial membrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome C into the cytosol, which in turn triggers other downstream events in the apoptotic cascade. Our Cell Meter™ NIR Mitochondria Membrane Potential Detection Kit provides all the essential components with an optimized assay method for the detection of apoptosis in cells with the loss of mitochondrial membrane potential. This fluorometric assay is based on the detection of the mitochondrial membrane potential in cells by our proprietary cationic MitoLite NIR™ dye. In normal cells, MitoLite NIR™ accumulates primarily in mitochondria, however, in apoptotic cells, MitoLite NIR™ staining intensity decreases. Cells stained with MitoLite NIR™ can be monitored fluorimetrically at 660-680 nm with excitation of 620-640 nm. The kit can be used for screening of apoptosis activators and inhibitors. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
The decrease in MitoLite™ NIR fluorescence with the addition of FCCP in HeLa cells. HeLa cells were loaded with MitoLite™ NIR alone or in the presence of 20 µM FCCP for 15 minutes. The fluorescence intensity of MitoLite™ NIR was measured 30 minutes after adding assay buffer with a FlexStation™ microplate reader (Molecular Devices) at Ex/Em = 640/680 nm (Cutoff = 665 nm, bottom read mode).
The decrease in MitoLite™ NIR fluorescence with the addition of FCCP in HeLa cells. HeLa cells were loaded with MitoLite™ NIR alone or in the presence of 20 µM FCCP for 15 minutes. The fluorescence intensity of MitoLite™ NIR was measured 30 minutes after adding assay buffer with a FlexStation™ microplate reader (Molecular Devices) at Ex/Em = 640/680 nm (Cutoff = 665 nm, bottom read mode).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22803500 Tests
Price
 
Spectral properties
Excitation (nm)640
Emission (nm)657
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Instrument settings
Fluorescence microplate reader
Excitation640 nm
Emission680 nm
Cutoff665 nm
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode
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Page updated on September 25, 2025