Name: CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm*
Brand: AAT Bioquest
SKU: 21903
Price: 208 USD
Availability: InStock

CytoCalcein™ Violet 660, sodium salt

Excited at 405 nm

CytoCalcein™ Violet 660, sodium salt is a membrane-impermeant violet-excited fluorescent dye corresponding to the esterase-cleaved form of CytoCalcein™ Violet 660 AM.

Violet laser excitation: Excited by the 405 nm violet laser for flow cytometry and compatible fluorescence microscopy workflows
Far-red emission profile: Provides violet-excited fluorescence with emission near 660 nm for expanded multiplexing options
Reference dye format: Serves as the esterase-cleaved reference product for CytoCalcein™ Violet 660 AM
Membrane-impermeant form: Represents the non-cell-permeable fluorescent form generated after intracellular hydrolysis of the AM derivative
Multipurpose applications: Suitable for assay calibration, reference controls, and fluorescence workflows requiring spectral separation from common fluorophores

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
21903	1 mg	Price

Overview

CytoCalcein™ Violet 660, sodium salt is the membrane-impermeant fluorescent form corresponding to the esterase-cleaved product of CytoCalcein™ Violet 660, AM. It is used as a reference dye for CytoCalcein™ Violet 660 AM, which is designed for live-cell labeling through intracellular esterase conversion in a manner similar to Calcein, AM.

CytoCalcein™ Violet 660 is excited by the 405 nm violet laser commonly available on modern flow cytometers and provides far-red emission near 660 nm. This violet-excited, far-red emission profile provides spectral separation from many commonly used flow cytometry and imaging fluorophores, supporting multiplexed fluorescence analysis, assay setup, and reference control workflows.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	150.914 µL	754.569 µL	1.509 mL	7.546 mL	15.091 mL
5 mM	30.183 µL	150.914 µL	301.828 µL	1.509 mL	3.018 mL
10 mM	15.091 µL	75.457 µL	150.914 µL	754.569 µL	1.509 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	662.63 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

A Ratiometric Probe Based on Carbon Dots and Calcein & Eu3+ for the Fluorescent Detection of Sodium Tripolyphosphate.

Authors:

Zheng, Yabin and Li, Chen and Li, Quan and Zhang, Taoyi and Chen, Jing and Ji, Wenxi and Wei, Yun

Journal:

Journal of fluorescence (2023): 965-972

Dual-emission fluorescence detection of histidine using carbon dots and calcein/Ni2+ complexes.

Authors:

Liu, Jinshui and Song, Bo and Fu, Ting

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 121951

Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen.

Authors:

Qi, Haiyan and Li, Qiuying and Jing, Jing and Jing, Tao and Liu, Chuntong and Qiu, Lixin and Sami, Rokayya and Helal, Mahmoud and Ismail, Khadiga Ahmed and Aljahani, Amani H

Journal:

Nanomaterials (Basel, Switzerland) (2022)

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters.

Authors:

Hausig-Punke, Franziska and Richter, Friederike and Hoernke, Maria and Brendel, Johannes C and Traeger, Anja

Journal:

Macromolecular bioscience (2022): e2200167

Sensitive ratiometric fluorescence probe based on chitosan carbon dots and calcein for Alkaline phosphatase detection and bioimaging in cancer cells.

Authors:

Mu, Xiaowei and Jiang, Xue and Zhang, Yu and Liu, Xin and Zhang, Siqi and Wang, Wei and Huang, Yibing and Ma, Pinyi and Song, Daqian

Journal:

Analytica chimica acta (2021): 339163

Physical properties

Molecular weight	662.63
Solvent	DMSO

Spectral properties

Excitation (nm)	402
Emission (nm)	660

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on August 29, 2026