CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 662.63 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | ![]() ![]() |
See also: Calcein AM Viability Dyes and Assay Kits
Molecular weight 662.63 |
CytoCalcein™ Violet 660 is the esterase-cleaved product of CytoCalcein™ Violet 660 AM in live cells. CytoCalcein™ Violet 660 is not cell-permemable. It is the reference product for the CytoCalcein™ Violet 660 AM that is designed for labeling live cells in the same way as Calcein, AM does. CytoCalcein™ Violet 660 is well excited with the Violet laser at 405 nm, which is now equipped in most flow cytometers. It is also well-excited by the excitation sources of fluorescence microscopes. This exceptional spectral separation from the typical existing FACS fluorophores provides additional options for multiplexing experiments with a flow cytometer or a fluorescence microscope. CytoCalcein™ Violet 660 has been developed for multiplexing flow cytometric and fluorescence imaging applications. It provides a new unique color for flow cytometric analysis of live cells.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 150.914 µL | 754.569 µL | 1.509 mL | 7.546 mL | 15.091 mL |
5 mM | 30.183 µL | 150.914 µL | 301.828 µL | 1.509 mL | 3.018 mL |
10 mM | 15.091 µL | 75.457 µL | 150.914 µL | 754.569 µL | 1.509 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
References
View all 50 references: Citation Explorer
A Ratiometric Probe Based on Carbon Dots and Calcein & Eu3+ for the Fluorescent Detection of Sodium Tripolyphosphate.
Authors: Zheng, Yabin and Li, Chen and Li, Quan and Zhang, Taoyi and Chen, Jing and Ji, Wenxi and Wei, Yun
Journal: Journal of fluorescence (2023): 965-972
Authors: Zheng, Yabin and Li, Chen and Li, Quan and Zhang, Taoyi and Chen, Jing and Ji, Wenxi and Wei, Yun
Journal: Journal of fluorescence (2023): 965-972
Dual-emission fluorescence detection of histidine using carbon dots and calcein/Ni2+ complexes.
Authors: Liu, Jinshui and Song, Bo and Fu, Ting
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 121951
Authors: Liu, Jinshui and Song, Bo and Fu, Ting
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 121951
Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen.
Authors: Qi, Haiyan and Li, Qiuying and Jing, Jing and Jing, Tao and Liu, Chuntong and Qiu, Lixin and Sami, Rokayya and Helal, Mahmoud and Ismail, Khadiga Ahmed and Aljahani, Amani H
Journal: Nanomaterials (Basel, Switzerland) (2022)
Authors: Qi, Haiyan and Li, Qiuying and Jing, Jing and Jing, Tao and Liu, Chuntong and Qiu, Lixin and Sami, Rokayya and Helal, Mahmoud and Ismail, Khadiga Ahmed and Aljahani, Amani H
Journal: Nanomaterials (Basel, Switzerland) (2022)
Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters.
Authors: Hausig-Punke, Franziska and Richter, Friederike and Hoernke, Maria and Brendel, Johannes C and Traeger, Anja
Journal: Macromolecular bioscience (2022): e2200167
Authors: Hausig-Punke, Franziska and Richter, Friederike and Hoernke, Maria and Brendel, Johannes C and Traeger, Anja
Journal: Macromolecular bioscience (2022): e2200167
Sensitive ratiometric fluorescence probe based on chitosan carbon dots and calcein for Alkaline phosphatase detection and bioimaging in cancer cells.
Authors: Mu, Xiaowei and Jiang, Xue and Zhang, Yu and Liu, Xin and Zhang, Siqi and Wang, Wei and Huang, Yibing and Ma, Pinyi and Song, Daqian
Journal: Analytica chimica acta (2021): 339163
Authors: Mu, Xiaowei and Jiang, Xue and Zhang, Yu and Liu, Xin and Zhang, Siqi and Wang, Wei and Huang, Yibing and Ma, Pinyi and Song, Daqian
Journal: Analytica chimica acta (2021): 339163
Calcein Binding to Assess Mineralization in Hydrogel Microspheres.
Authors: White, Kristopher and Chalaby, Rabab and Lowe, Gina and Berlin, Jacob and Glackin, Carlotta and Olabisi, Ronke
Journal: Polymers (2021)
Authors: White, Kristopher and Chalaby, Rabab and Lowe, Gina and Berlin, Jacob and Glackin, Carlotta and Olabisi, Ronke
Journal: Polymers (2021)
Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay.
Authors: Lorenzo-Herrero, Seila and Sordo-Bahamonde, Christian and González, Segundo and López-Soto, Alejandro
Journal: Methods in enzymology (2020): 483-495
Authors: Lorenzo-Herrero, Seila and Sordo-Bahamonde, Christian and González, Segundo and López-Soto, Alejandro
Journal: Methods in enzymology (2020): 483-495
Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells.
Authors: Kitchen, Philip and Salman, Mootaz M and Abir-Awan, Mohammed and Al-Jubair, Tamim and Törnroth-Horsefield, Susanna and Conner, Alex C and Bill, Roslyn M
Journal: STAR protocols (2020): 100157
Authors: Kitchen, Philip and Salman, Mootaz M and Abir-Awan, Mohammed and Al-Jubair, Tamim and Törnroth-Horsefield, Susanna and Conner, Alex C and Bill, Roslyn M
Journal: STAR protocols (2020): 100157
Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein.
Authors: Dutta, Sayan and Watson, Ben G and Mattoo, Seema and Rochet, Jean-Christophe
Journal: Bio-protocol (2020)
Authors: Dutta, Sayan and Watson, Ben G and Mattoo, Seema and Rochet, Jean-Christophe
Journal: Bio-protocol (2020)
The Relationship Between Water Transmission Rate and Defects on the Film Based on the Defect Analysis Using Fluorescent Calcein Probe.
Authors: Kim, Sun Min and Kim, Hyun Chul and Kim, Chang-Yeoul and Yoon, Daeho and Koo, Eunhae
Journal: Journal of nanoscience and nanotechnology (2020): 5469-5472
Authors: Kim, Sun Min and Kim, Hyun Chul and Kim, Chang-Yeoul and Yoon, Daeho and Koo, Eunhae
Journal: Journal of nanoscience and nanotechnology (2020): 5469-5472