Name: CytoCalcein™ Violet 660, AM *Excited at 405 nm*
Brand: AAT Bioquest
SKU: 21904
Price: 208 USD
Availability: InStock

CytoCalcein™ Violet 660, AM

Excited at 405 nm

CytoCalcein™ Violet 660, AM Excited at 405 nm is a cell-permeant live-cell labeling dye that generates violet-excited far-red fluorescence after intracellular esterase hydrolysis.

Live-cell retention: Enters cells as a cell-permeant AM ester and is converted into a negatively charged fluorescent product retained in viable cells
Far-red emission profile: Provides violet-excited far-red fluorescence for expanded multiplexing options
Large Stokes shift: Provides strong separation between violet excitation and far-red emission for multiplexed fluorescence analysis
Multiplexing applications: Suitable for live-cell labeling in flow cytometry and fluorescence microscopy workflows

Protocol

SDS

Catalog	Size	Price	Quantity
21904	10x50 ug	Price

Overview

CytoCalcein™ Violet 660, AM is a cell-permeant AM ester dye designed for live-cell labeling in a manner similar to Calcein, AM. After entering live cells, the AM ester is hydrolyzed by intracellular esterases to generate a fluorescent, negatively charged form that is retained within viable cells.

CytoCalcein™ Violet 660, AM is excited by the 405 nm violet laser commonly available on modern flow cytometers and emits in the far-red region near 660 nm. Its large Stokes shift provides spectral separation from many commonly used flow cytometry and imaging fluorophores, supporting multiplexed live-cell analysis by flow cytometry and fluorescence microscopy.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, AM *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	123.021 µL	615.105 µL	1.23 mL	6.151 mL	12.302 mL
5 mM	24.604 µL	123.021 µL	246.042 µL	1.23 mL	2.46 mL
10 mM	12.302 µL	61.51 µL	123.021 µL	615.105 µL	1.23 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	812.87 g/mol	=	mL	x	mM	=	mmol

References

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He, Qi and Yang, Junzheng and Pan, Zhaofeng and Zhang, Gangyu and Chen, Baihao and Li, Shaocong and Xiao, Jiacong and Tan, Fengjin and Wang, Zihao and Chen, Peng and Wang, Haibin

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2023): 113915

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Yu, Xiaorong and Yang, Yunpeng and Zhang, Bo and Han, Guangyu and Yu, Junxing and Yu, Qi and Zhang, Lei

Journal:

Neuroscience (2023): 157-172

Effects of Moringa oleifera Seed Oil on Cultured Human Sebocytes In Vitro and Comparison with Other Oil Types.

Authors:

Zouboulis, Christos C and Hossini, Amir M and Hou, Xiaoxiao and Wang, Chaoxuan and Weylandt, Karsten H and Pietzner, Anne

Journal:

International journal of molecular sciences (2023)

Role of ferroptosis in hypoxic preconditioning to reduce propofol neurotoxicity.

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Chen, Jing and Xiao, Fei and Chen, Lifei and Zhou, Zhan and Wei, Yi and Zhong, Yu and Li, Li and Xie, Yubo

Journal:

Frontiers in pharmacology (2023): 1121280

Physical properties

Molecular weight	812.87
Solvent	DMSO

Spectral properties

Excitation (nm)	402
Emission (nm)	660

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	660/20 nm filter

Fluorescence microscope
Excitation	405 nm laser
Emission	675/30 nm
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	405
Emission	660
Cutoff	630
Recommended plate	Solid black

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Page updated on July 27, 2026