AAT Bioquest

CytoCalcein™ Violet 660, AM *Excited at 405 nm*

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Physical properties
Molecular weight812.87
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
CytoCalcein™ Violet 660 AM ester is designed for labeling live cells in the same way as Calcein, AM does. It is well excited with the Violet laser at 405 nm, which is now equipped in most flow cytometers. It is also well-excited by the excitation sources of fluorescence microscopes. Upon getting into live cells the CytoCalcein™ Violet 660 is hydrolyzed into a fluorescent dye of multiple negative charges, which makes the dye well retained in live cells. It has the largest Stokes Shift among the known live cell stains. This exceptional spectral separation from the typical existing FACS fluorophores provides additional options for multiplexing experiments with a flow cytometer or a fluorescence microscope. CytoCalcein™ Violet 660 has been developed for multiplexing flow cytometric and fluorescence imaging applications. It provides a new unique color for flow cytometric analysis of live cells.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, AM *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM123.021 µL615.105 µL1.23 mL6.151 mL12.302 mL
5 mM24.604 µL123.021 µL246.042 µL1.23 mL2.46 mL
10 mM12.302 µL61.51 µL123.021 µL615.105 µL1.23 mL

Molarity calculator

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