CytoCalcein™ Violet 660, AM *Excited at 405 nm*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 812.87 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
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See also: Calcein AM Viability Dyes and Assay Kits
Molecular weight 812.87 |
CytoCalcein™ Violet 660 AM ester is designed for labeling live cells in the same way as Calcein, AM does. It is well excited with the Violet laser at 405 nm, which is now equipped in most flow cytometers. It is also well-excited by the excitation sources of fluorescence microscopes. Upon getting into live cells the CytoCalcein™ Violet 660 is hydrolyzed into a fluorescent dye of multiple negative charges, which makes the dye well retained in live cells. It has the largest Stokes Shift among the known live cell stains. This exceptional spectral separation from the typical existing FACS fluorophores provides additional options for multiplexing experiments with a flow cytometer or a fluorescence microscope. CytoCalcein™ Violet 660 has been developed for multiplexing flow cytometric and fluorescence imaging applications. It provides a new unique color for flow cytometric analysis of live cells.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, AM *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 123.021 µL | 615.105 µL | 1.23 mL | 6.151 mL | 12.302 mL |
5 mM | 24.604 µL | 123.021 µL | 246.042 µL | 1.23 mL | 2.46 mL |
10 mM | 12.302 µL | 61.51 µL | 123.021 µL | 615.105 µL | 1.23 mL |
Molarity calculator
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References
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