CytoCalcein™ Violet 660, AM Excited at 405 nm

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Physical properties

Molecular weight	812.87
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

812.87

CytoCalcein™ Violet 660 AM ester is designed for labeling live cells in the same way as Calcein, AM does. It is well excited with the Violet laser at 405 nm, which is now equipped in most flow cytometers. It is also well-excited by the excitation sources of fluorescence microscopes. Upon getting into live cells the CytoCalcein™ Violet 660 is hydrolyzed into a fluorescent dye of multiple negative charges, which makes the dye well retained in live cells. It has the largest Stokes Shift among the known live cell stains. This exceptional spectral separation from the typical existing FACS fluorophores provides additional options for multiplexing experiments with a flow cytometer or a fluorescence microscope. CytoCalcein™ Violet 660 has been developed for multiplexing flow cytometric and fluorescence imaging applications. It provides a new unique color for flow cytometric analysis of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, AM *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	123.021 µL	615.105 µL	1.23 mL	6.151 mL	12.302 mL
5 mM	24.604 µL	123.021 µL	246.042 µL	1.23 mL	2.46 mL
10 mM	12.302 µL	61.51 µL	123.021 µL	615.105 µL	1.23 mL

Molarity calculator

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