CytoTrace™ CM-DiD
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1077.55 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 260000 |
Excitation (nm) | 643 |
Emission (nm) | 663 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | ![]() ![]() |
Molecular weight 1077.55 | Extinction coefficient (cm -1 M -1) 260000 | Excitation (nm) 643 | Emission (nm) 663 |
CM-DiD is the longer wavelength analog of 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine iodide (CM-DiI). It is a lipophilic carbocyanine dye that can be used for labeling cell membranes in various biological and neuroscientific research applications. Compared to the well-known CM-DiI, CM-DiD has much longer excitation and emission wavelengths. CM-DiD is compatible with the Cy5 filter set. It is well excited with the red laser at 647 nm or He-Ne laser at 633 nm. It is a fluorescent dye that exhibits strong fluorescence properties when incorporated into cell membranes or lipid-containing structures. CM-DiI can be used to label and trace cell membranes to study cell migration, cell tracking, and axonal projections in live and fixed tissues. Once incorporated into the cell membrane, CM-DiD tends to stay in place and does not rapidly diffuse within the membrane. This characteristic enables long-term imaging and tracing studies. In neuroscience, CM-DiD might be particularly valuable for studying neural connections and pathways. CM-DiD is relatively stable, and its fluorescence is resistant to photobleaching, allowing for extended imaging sessions without significant loss of signal. It allows researchers to visualize and analyze labeled structures with high specificity. CM-DiD can be used in both cell cultures and living organisms, allowing researchers to study biological processes at various levels of complexity.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ CM-DiD to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 92.803 µL | 464.016 µL | 928.031 µL | 4.64 mL | 9.28 mL |
5 mM | 18.561 µL | 92.803 µL | 185.606 µL | 928.031 µL | 1.856 mL |
10 mM | 9.28 µL | 46.402 µL | 92.803 µL | 464.016 µL | 928.031 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Extinction coefficient (cm -1 M -1) | 260000 |
Excitation (nm) | 643 |
Emission (nm) | 663 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
CytoTrace™ CM-DiI | 548 | 563 | 148000 |
Images
Figure 1. CM-DiD is the longer wavelength analog of 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine iodide (CM-DiI). It is a lipophilic carbocyanine dye that can be used for labeling cell membranes in various biological and neuroscientific research applications. Compared to the well-known CM-DiI, CM-DiD has much longer excitation and emission wavelengths. CM-DiD is compatible with the Cy5 filter set.
References
View all 50 references: Citation Explorer
Evaluation of cell disruption technologies on magnetosome chain length and aggregation behaviour from Magnetospirillum gryphiswaldense MSR-1.
Authors: Masó-Martínez, Marta and Fryer, Benjamin and Aubert, Dimitri and Peacock, Benjamin and Lees, Rebecca and Rance, Graham A and Fay, Michael W and Topham, Paul D and Fernández-Castané, Alfred
Journal: Frontiers in bioengineering and biotechnology (2023): 1172457
Authors: Masó-Martínez, Marta and Fryer, Benjamin and Aubert, Dimitri and Peacock, Benjamin and Lees, Rebecca and Rance, Graham A and Fay, Michael W and Topham, Paul D and Fernández-Castané, Alfred
Journal: Frontiers in bioengineering and biotechnology (2023): 1172457
Whole-Slide Imaging, Mutual Information Registration for Multiplex Immunohistochemistry and Immunofluorescence.
Authors: Doyle, Joshua and Green, Benjamin F and Eminizer, Margaret and Jimenez-Sanchez, Daniel and Lu, Steve and Engle, Elizabeth L and Xu, Haiying and Ogurtsova, Aleksandra and Lai, Jonathan and Soto-Diaz, Sigfredo and Roskes, Jeffrey S and Deutsch, Julie S and Taube, Janis M and Sunshine, Joel C and Szalay, Alexander S
Journal: Laboratory investigation; a journal of technical methods and pathology (2023): 100175
Authors: Doyle, Joshua and Green, Benjamin F and Eminizer, Margaret and Jimenez-Sanchez, Daniel and Lu, Steve and Engle, Elizabeth L and Xu, Haiying and Ogurtsova, Aleksandra and Lai, Jonathan and Soto-Diaz, Sigfredo and Roskes, Jeffrey S and Deutsch, Julie S and Taube, Janis M and Sunshine, Joel C and Szalay, Alexander S
Journal: Laboratory investigation; a journal of technical methods and pathology (2023): 100175
Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain.
Authors: Liu, Tianyan and Stephan, Till and Chen, Peng and Keller-Findeisen, Jan and Chen, Jingting and Riedel, Dietmar and Yang, Zhongtian and Jakobs, Stefan and Chen, Zhixing
Journal: Proceedings of the National Academy of Sciences of the United States of America (2022): e2215799119
Authors: Liu, Tianyan and Stephan, Till and Chen, Peng and Keller-Findeisen, Jan and Chen, Jingting and Riedel, Dietmar and Yang, Zhongtian and Jakobs, Stefan and Chen, Zhixing
Journal: Proceedings of the National Academy of Sciences of the United States of America (2022): e2215799119
Potassium permanganate is an excellent alternative to osmium tetroxide in freeze-substitution.
Authors: Schauflinger, Martin and Bergner, Tim and Neusser, Gregor and Kranz, Christine and Read, Clarissa
Journal: Histochemistry and cell biology (2022): 481-489
Authors: Schauflinger, Martin and Bergner, Tim and Neusser, Gregor and Kranz, Christine and Read, Clarissa
Journal: Histochemistry and cell biology (2022): 481-489
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder.
Authors: Segars, Kristen L and Azzari, Nicholas A and Gomez, Stephanie and Rich, Celeste B and Trinkaus-Randall, Vickery
Journal: Journal of visualized experiments : JoVE (2022)
Authors: Segars, Kristen L and Azzari, Nicholas A and Gomez, Stephanie and Rich, Celeste B and Trinkaus-Randall, Vickery
Journal: Journal of visualized experiments : JoVE (2022)
A simple method to isolate term trophoblasts and maintain them in extended culture.
Authors: Nursalim, Y N S and Groom, K M and Blenkiron, C and Chamley, L W
Journal: Placenta (2021): 1-10
Authors: Nursalim, Y N S and Groom, K M and Blenkiron, C and Chamley, L W
Journal: Placenta (2021): 1-10
Vitrectomy for the removal of idiopathic epiretinal membrane with or without internal limiting membrane peeling: a meta-analysis.
Authors: Christodoulou, Eleni and Batsos, Georgios and Galanis, Petros and Kalogeropoulos, Christos and Katsanos, Andreas and Alamanos, Yannis and Stefaniotou, Maria
Journal: Therapeutic advances in ophthalmology (2020): 2515841420927133
Authors: Christodoulou, Eleni and Batsos, Georgios and Galanis, Petros and Kalogeropoulos, Christos and Katsanos, Andreas and Alamanos, Yannis and Stefaniotou, Maria
Journal: Therapeutic advances in ophthalmology (2020): 2515841420927133
Highly Fluorescent Distyrylnaphthalene Derivatives as a Tool for Visualization of Cellular Membranes.
Authors: Suwara, Justyna and Lukasik, Beata and Zurawinski, Remigiusz and Pawlowska, Roza and Chworos, Arkadiusz
Journal: Materials (Basel, Switzerland) (2020)
Authors: Suwara, Justyna and Lukasik, Beata and Zurawinski, Remigiusz and Pawlowska, Roza and Chworos, Arkadiusz
Journal: Materials (Basel, Switzerland) (2020)
Therapeutic Potential of Two Visible Light Responsive Luminescent photoCORMs: Enhanced Cellular Internalization Driven by Lipophilicity.
Authors: Pinto, Miguel N and Chakraborty, Indranil and Jimenez, Jorge and Murphy, Katelyn and Wenger, John and Mascharak, Pradip K
Journal: Inorganic chemistry (2019): 14522-14531
Authors: Pinto, Miguel N and Chakraborty, Indranil and Jimenez, Jorge and Murphy, Katelyn and Wenger, John and Mascharak, Pradip K
Journal: Inorganic chemistry (2019): 14522-14531
Quantifying Heterogeneity of Individual Organelles in Mixed Populations via Mass Cytometry.
Authors: Brown, Heather M G and Arriaga, Edgar A
Journal: Analytical chemistry (2018): 13315-13321
Authors: Brown, Heather M G and Arriaga, Edgar A
Journal: Analytical chemistry (2018): 13315-13321