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CytoTrace™ CM-DiD

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Physical properties
Molecular weight1077.55
Spectral properties
Extinction coefficient (cm -1 M -1)260000
Excitation (nm)643
Emission (nm)663
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
CM-DiD is the longer wavelength analog of 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine iodide (CM-DiI). It is a lipophilic carbocyanine dye that can be used for labeling cell membranes in various biological and neuroscientific research applications. Compared to the well-known CM-DiI, CM-DiD has much longer excitation and emission wavelengths. CM-DiD is compatible with the Cy5 filter set. It is well excited with the red laser at 647 nm or He-Ne laser at 633 nm. It is a fluorescent dye that exhibits strong fluorescence properties when incorporated into cell membranes or lipid-containing structures. CM-DiI can be used to label and trace cell membranes to study cell migration, cell tracking, and axonal projections in live and fixed tissues. Once incorporated into the cell membrane, CM-DiD tends to stay in place and does not rapidly diffuse within the membrane. This characteristic enables long-term imaging and tracing studies. In neuroscience, CM-DiD might be particularly valuable for studying neural connections and pathways. CM-DiD is relatively stable, and its fluorescence is resistant to photobleaching, allowing for extended imaging sessions without significant loss of signal. It allows researchers to visualize and analyze labeled structures with high specificity. CM-DiD can be used in both cell cultures and living organisms, allowing researchers to study biological processes at various levels of complexity.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ CM-DiD to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM92.803 µL464.016 µL928.031 µL4.64 mL9.28 mL
5 mM18.561 µL92.803 µL185.606 µL928.031 µL1.856 mL
10 mM9.28 µL46.402 µL92.803 µL464.016 µL928.031 µL

Molarity calculator

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Spectral properties

Extinction coefficient (cm -1 M -1)260000
Excitation (nm)643
Emission (nm)663

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
CytoTrace™ CM-DiI548563148000



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