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CytoTrace™ CM-DiI

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Physical properties
Molecular weight1051.51
Spectral properties
Extinction coefficient (cm -1 M -1)148000
Excitation (nm)548
Emission (nm)563
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine iodide (CM-DiI) is a lipophilic carbocyanine dye that is commonly used for labeling cell membranes in various biological and neuroscientific research applications. It is a fluorescent dye that exhibits strong fluorescence properties when incorporated into cell membranes or lipid-containing structures. CM-DiI is widely used to label and trace cell membranes to study cell migration, cell tracking, and axonal projections in live and fixed tissues. Once incorporated into the cell membrane, CM-DiI tends to stay in place and does not rapidly diffuse within the membrane. This characteristic enables long-term imaging and tracing studies. In neuroscience, CM-DiI is particularly valuable for studying neural connections and pathways. Researchers can apply CM-DiI to specific regions of the brain or spinal cord and observe the labeled axons projecting to other regions. CM-DiI emits strong red fluorescence, making it easily detectable and suitable for fluorescence microscopy. CM-DiI is relatively stable, and its fluorescence is resistant to photobleaching, allowing for extended imaging sessions without significant loss of signal. It allows researchers to visualize and analyze labeled structures with high specificity. CM-DiI can be used in both cell cultures and living organisms, allowing researchers to study biological processes at various levels of complexity.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ CM-DiI to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM95.101 µL475.507 µL951.013 µL4.755 mL9.51 mL
5 mM19.02 µL95.101 µL190.203 µL951.013 µL1.902 mL
10 mM9.51 µL47.551 µL95.101 µL475.507 µL951.013 µL

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Spectral properties

Extinction coefficient (cm -1 M -1)148000
Excitation (nm)548
Emission (nm)563

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
CytoTrace™ CM-DiD643663260000



View all 3 references: Citation Explorer
CLARITY-compatible lipophilic dyes for electrode marking and neuronal tracing.
Authors: Jensen, Kristian H R and Berg, Rune W
Journal: Scientific reports (2016): 32674
Visualization of interstitial cells of Cajal in living, intact tissues.
Authors: Hanani, M and Belzer, V and Rich, A and Faussone-Pellegrini, S M
Journal: Microscopy research and technique (1999): 336-43
Visualization of interstitial cells of Cajal in the mouse colon by vital staining.
Authors: Hanani, M and Louzon, V and Miller, S M and Faussone-Pellegrini, M S
Journal: Cell and tissue research (1998): 275-82