DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide]
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 546.53 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Cell/Cytoplasmic Membrane Potential Activity & Analysis, Plasma Membrane, Membrane Potential and Channels, Neurodegeneration & Amyloid Staining
Molecular weight 546.53 |
DiI, DiO, DiS, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 182.973 µL | 914.863 µL | 1.83 mL | 9.149 mL | 18.297 mL |
5 mM | 36.595 µL | 182.973 µL | 365.945 µL | 1.83 mL | 3.659 mL |
10 mM | 18.297 µL | 91.486 µL | 182.973 µL | 914.863 µL | 1.83 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-SFX [5-FAM-X, SE] *CAS 148356-00-7* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7* | 491 | 516 | 73000 | - | 0.35 |
5-DTAF [5-(4,6-Dichlorotriazinyl)aminofluorescein] *CAS 51306-35-5* | 498 | 517 | 800001 | 0.32 | 0.35 |
5-IAF [5-Iodoacetamidofluorescein] *CAS 63368-54-7* | 498 | 517 | 800001 | 0.32 | 0.35 |
5-CR110 [5-Carboxyrhodamine 110] *Single isomer* | 499 | 525 | 84000 | 0.24 | 0.091 |
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer* | 522 | 546 | 94000 | 0.24 | 0.214 |
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4* | 552 | 578 | 90000 | 0.32 | 0.178 |
5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3* | 578 | 604 | 82000 | - | 0.168 |
Show More (3) |
Citations
View all 51 citations: Citation Explorer
A Novel Two-Component Bacteriocin, Acidicin P, and Its Key Residues for Inhibiting Listeria monocytogenes by Targeting the Cell Membrane
Authors: Xia, Tianqi and Teng, Kunling and Liu, Yayong and Guo, Yaoqi and Huang, Fuqing and Tahir, Muhammad and Wang, Tianwei and Zhong, Jin
Journal: Microbiology Spectrum (2023): e05210--22
Authors: Xia, Tianqi and Teng, Kunling and Liu, Yayong and Guo, Yaoqi and Huang, Fuqing and Tahir, Muhammad and Wang, Tianwei and Zhong, Jin
Journal: Microbiology Spectrum (2023): e05210--22
The enhancement effect of small molecule Lyb24 reveals AzoR as a novel target of polymyxin B
Authors: Hu, Chunxia and Zhang, Jinyong and Cui, Ruiqin and Liu, Shiyi and Huang, Ying and Zeng, Huan and Cheng, Shumin and Zhou, Guibao and Li, Jingli and Sun, Longqin and others,
Journal: Biomedicine \& Pharmacotherapy (2023): 115856
Authors: Hu, Chunxia and Zhang, Jinyong and Cui, Ruiqin and Liu, Shiyi and Huang, Ying and Zeng, Huan and Cheng, Shumin and Zhou, Guibao and Li, Jingli and Sun, Longqin and others,
Journal: Biomedicine \& Pharmacotherapy (2023): 115856
Anti-hepatitis C virus drug simeprevir: a promising antimicrobial agent against MRSA
Authors: Li, Yimin and She, Pengfei and Xu, Lanlan and Liu, Yaqian and Liu, Shasha and Li, Zehao and Yang, Yifan and Li, Linhui and Hussain, Zubair and Wu, Yong
Journal: Applied Microbiology and Biotechnology (2022): 2689--2702
Authors: Li, Yimin and She, Pengfei and Xu, Lanlan and Liu, Yaqian and Liu, Shasha and Li, Zehao and Yang, Yifan and Li, Linhui and Hussain, Zubair and Wu, Yong
Journal: Applied Microbiology and Biotechnology (2022): 2689--2702
The determination of antibacterial mode for cationic lipopeptides brevibacillins against Salmonella typhimurium by quantum chemistry calculation
Authors: Wu, Yubo and Nie, Ting and Meng, Fanqiang and Zhou, Libang and Chen, Meirong and Sun, Jing and Lu, Zhaoxin and Lu, Yingjian
Journal: Applied Microbiology and Biotechnology (2021): 1--13
Authors: Wu, Yubo and Nie, Ting and Meng, Fanqiang and Zhou, Libang and Chen, Meirong and Sun, Jing and Lu, Zhaoxin and Lu, Yingjian
Journal: Applied Microbiology and Biotechnology (2021): 1--13
Genetic engineering of circularly permuted yellow fluorescent protein reveals intracellular acidification in response to nitric oxide stimuli
Authors: Deng, Haitao and Li, Jingyi and Zhou, Yao and Xia, Yang and Chen, Chao and Zhou, Zhemin and Wu, Hui and Wang, Ping and Zhou, Shengmin
Journal: Redox biology (2021): 101943
Authors: Deng, Haitao and Li, Jingyi and Zhou, Yao and Xia, Yang and Chen, Chao and Zhou, Zhemin and Wu, Hui and Wang, Ping and Zhou, Shengmin
Journal: Redox biology (2021): 101943
Co3O4 Nanowires Capable of Discharging Low Voltage Electricity Showing Potent Antibacterial Activity for Treatment of Bacterial Skin Infection
Authors: Li, Chengnan and Li, Zongshao and Zeng, Yinxiang and Cao, Xianshuo and Zhao, Huimin and Yang, Yi Yan and Yuan, Peiyan and Lu, Xihong and Ding, Xin
Journal: Advanced healthcare materials (2021): 2102044
Authors: Li, Chengnan and Li, Zongshao and Zeng, Yinxiang and Cao, Xianshuo and Zhao, Huimin and Yang, Yi Yan and Yuan, Peiyan and Lu, Xihong and Ding, Xin
Journal: Advanced healthcare materials (2021): 2102044
Repurposing antispasmodic agent otilonium bromide for treatment of Staphylococcus aureus infections
Authors: Zhou, Linying and She, Pengfei and Tan, Fang and Li, Shijia and Zeng, Xianghai and Chen, Lihua and Luo, Zhen and Wu, Yong
Journal: Frontiers in microbiology (2020): 1720
Authors: Zhou, Linying and She, Pengfei and Tan, Fang and Li, Shijia and Zeng, Xianghai and Chen, Lihua and Luo, Zhen and Wu, Yong
Journal: Frontiers in microbiology (2020): 1720
The antibacterial activity and mechanism of action of luteolin against Trueperella pyogenes
Authors: Guo, Yuru and Liu, Yan and Zhang, Zehui and Chen, Menghan and Zhang, Dexian and Tian, Chunlian and Liu, Mingchun and Jiang, Guotuo
Journal: Infection and Drug Resistance (2020): 1697
Authors: Guo, Yuru and Liu, Yan and Zhang, Zehui and Chen, Menghan and Zhang, Dexian and Tian, Chunlian and Liu, Mingchun and Jiang, Guotuo
Journal: Infection and Drug Resistance (2020): 1697
Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
The accelerated blood clearance phenomenon of PEGylated nanoemulsion upon cross administration with nanoemulsions modified with polyglycerin
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
References
View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Application notes
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Introducing Calbryte™ Series
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Introducing Calbryte™ Series