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DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide]

Chemical structure for DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide]
Chemical structure for DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide]
Ordering information
Price ()
Catalog Number22076
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight546.53
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
DiI, DiO, DiS, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM182.973 µL914.863 µL1.83 mL9.149 mL18.297 mL
5 mM36.595 µL182.973 µL365.945 µL1.83 mL3.659 mL
10 mM18.297 µL91.486 µL182.973 µL914.863 µL1.83 mL

Molarity calculator

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Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
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Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
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In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
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Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
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Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
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Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
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Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
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