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DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide]

DiI, DiO, DiS, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiSC3(5) [3,3-Dipropylthiadicarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM182.973 µL914.863 µL1.83 mL9.149 mL18.297 mL
5 mM36.595 µL182.973 µL365.945 µL1.83 mL3.659 mL
10 mM18.297 µL91.486 µL182.973 µL914.863 µL1.83 mL

Molarity calculator

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Citations

View all 54 citations: Citation Explorer
Synergistic collaboration between AMPs and non-direct antimicrobial cationic peptides
Authors: Ye, Zifan and Fu, Lei and Li, Shuangyu and Chen, Ziying and Ouyang, Jianhong and Shang, Xinci and Liu, Yanli and Gao, Lianghui and Wang, Yipeng
Journal: Nature Communications (2024): 7319
Flagellar pH homeostasis mediated by Na+/H+ exchangers regulates human sperm functions through coupling with CatSper and KSper activation
Authors: Liang, Min and Ji, Nanxi and Song, Jian and Kang, Hang and Zeng, Xuhui
Journal: Human Reproduction (2024): deae020
The enhancement effect of small molecule Lyb24 reveals AzoR as a novel target of polymyxin B
Authors: Hu, Chunxia and Zhang, Jinyong and Cui, Ruiqin and Liu, Shiyi and Huang, Ying and Zeng, Huan and Cheng, Shumin and Zhou, Guibao and Li, Jingli and Sun, Longqin and others,
Journal: Biomedicine \& Pharmacotherapy (2023): 115856
A Novel Two-Component Bacteriocin, Acidicin P, and Its Key Residues for Inhibiting Listeria monocytogenes by Targeting the Cell Membrane
Authors: Xia, Tianqi and Teng, Kunling and Liu, Yayong and Guo, Yaoqi and Huang, Fuqing and Tahir, Muhammad and Wang, Tianwei and Zhong, Jin
Journal: Microbiology Spectrum (2023): e05210--22

References

View all 92 references: Citation Explorer
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Page updated on October 4, 2024

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Catalog Number22076
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Physical properties

Molecular weight

546.53

Solvent

DMSO

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200