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EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*

EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM375.488 µL1.877 mL3.755 mL18.774 mL37.549 mL
5 mM75.098 µL375.488 µL750.976 µL3.755 mL7.51 mL
10 mM37.549 µL187.744 µL375.488 µL1.877 mL3.755 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
ICG acid789813230000-0.076-
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.0730.07
Cy5NS acid65167025000010.271, 0.420.030.02
Cy7NS acid7567792500000.30.0360.05
AMCA acid *CAS#: 106562-32-7*34643419000-0.153-
EDANS iodoacetamide [5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid]3364555900-0.107-
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*----0.516-
BCECF acid *CAS#: 85138-49-4*50452790000---
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.090.24
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.110.30
Cy3B acid56057112000010.5810.0690.048
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.190.25
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.7910.120.21
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.460.45
XFD514 acid51854380000-0.180.31
QXY7 acid [equivalent to QSY-7 acid]--900001-0.22-
XFD555 acid5535681550000.110.080.08
XFD647 acid6506712700000.3310.03-
XFD750 acid7527762900000.1210.04-
XFD700 acid6967192050000.2510.07-
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]51353385000-0.19-
XFD405 acid40142135,000-0.700.23
XFD430 acid43254015,000-0.28-
Show More (14)

References

View all 54 references: Citation Explorer
The beta4-beta8 Groove Is an ATP-interactive Site in the alpha Crystallin Core Domain of the Small Heat Shock Protein, Human alphaB Crystallin
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Page updated on October 4, 2024

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Physical properties

Molecular weight

266.32

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.107

Extinction coefficient (cm -1 M -1)

5900

Excitation (nm)

336

Emission (nm)

455

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

50402-56-7