EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]
| Overview |  Protocol |
See also: Cell Structures and Organelles, Nucleus, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
CAS 50402-56-7 | Molecular weight 266.32 | Correction Factor (280 nm) 0.107 | Extinction coefficient (cm -1 M -1) 5900 | Excitation (nm) 336 | Emission (nm) 455 |
EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 375.488 µL | 1.877 mL | 3.755 mL | 18.774 mL | 37.549 mL |
| 5 mM | 75.098 µL | 375.488 µL | 750.976 µL | 3.755 mL | 7.51 mL |
| 10 mM | 37.549 µL | 187.744 µL | 375.488 µL | 1.877 mL | 3.755 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
| Correction Factor (280 nm) | 0.107 |
| Extinction coefficient (cm -1 M -1) | 5900 |
| Excitation (nm) | 336 |
| Emission (nm) | 455 |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| ICG acid | 789 | 814 | - | - | 0.076 |
| Cy3NS acid *CAS 1032678-01-5* | 555 | 569 | 1500001 | 0.151 | 0.073 |
| Cy5NS acid | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 |
| Cy7NS acid | 756 | 779 | 250000 | 0.3 | 0.036 |
| AMCA acid *CAS#: 106562-32-7* | 346 | 434 | 19000 | - | 0.153 |
| EDANS iodoacetamide [5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid] | 336 | 455 | 5900 | - | 0.107 |
| DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* | - | - | - | - | 0.516 |
| BCECF acid *CAS#: 85138-49-4* | 504 | 527 | 90000 | - | - |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.611 | - |
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References
View all 54 references: Citation Explorer
The beta4-beta8 Groove Is an ATP-interactive Site in the alpha Crystallin Core Domain of the Small Heat Shock Protein, Human alphaB Crystallin
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
Authors: Szabelski M, Rogiewicz M, Wiczk W.
Journal: Anal Biochem (2005): 20
Authors: Szabelski M, Rogiewicz M, Wiczk W.
Journal: Anal Biochem (2005): 20
ADAM33 enzyme properties and substrate specificity
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247
Chemical synthesis of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein
Authors: Engfeldt T, Renberg B, Brumer H, Nygren PA, Karlstrom AE.
Journal: Chembiochem (2005): 1043
Authors: Engfeldt T, Renberg B, Brumer H, Nygren PA, Karlstrom AE.
Journal: Chembiochem (2005): 1043
Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99
On the sequential determinants of calpain cleavage
Authors: Tompa P, Buzder-Lantos P, Tantos A, Farkas A, Szilagyi A, Banoczi Z, Hudecz F, Friedrich P.
Journal: J Biol Chem (2004): 20775
Authors: Tompa P, Buzder-Lantos P, Tantos A, Farkas A, Szilagyi A, Banoczi Z, Hudecz F, Friedrich P.
Journal: J Biol Chem (2004): 20775