Fura-8™, pentapotassium salt
Although Fura-2 has become the preferred excitation-ratioable calcium indicator of choice, it has certain limitations (e.g., lower sensitivity than single wavelength calcium indicators such as Fluo-8® and Cal-520®). To address these concerns, AAT Bioquest has devoted considerable efforts to the development of Fura™ 8, a high-affinity ratiometric calcium indicator with improved sensitivity and higher signal-to-noise ratios. The fluorescence emission of Fura™ 8 is red-shifted to a longer visible wavelength, facilitating the detection of Fura™ 8 by common filter sets. Fura™ 8, pentapotassium salt is membrane-impermeant and is excited at 355 nm and 415 nm and emits at 530 nm.
![Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ffura-8-pentapotassium-salt%2Ffigure-for-fura-8-pentapotassium-salt_tGJgQ.jpg&w=640&q=75)
![Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ffura-8-pentapotassium-salt%2Ffigure-for-fura-8-pentapotassium-salt_tGJgQ.jpg&w=640&q=75)
![Fluorescence excitation spectra of Fura-8™ in the presence of 0 to 39 µM free Ca2+.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Ffura-8-pentapotassium-salt%2Ffigure-for-fura-8-pentapotassium-salt_tGJgQ.jpg&w=128&q=25)
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fura-8™, pentapotassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 122.548 µL | 612.738 µL | 1.225 mL | 6.127 mL | 12.255 mL |
5 mM | 24.51 µL | 122.548 µL | 245.095 µL | 1.225 mL | 2.451 mL |
10 mM | 12.255 µL | 61.274 µL | 122.548 µL | 612.738 µL | 1.225 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
Name | Excitation (nm) | Emission (nm) |
Fura-8FF™, pentapotassium salt | 354 | 524 |
Fura-8™, pentasodium salt | 354 | 524 |
Fura-2, pentapotassium salt *CAS 113694-64-7* | 336 | 505 |
Fura-FF, pentapotassium salt [Fura-2FF, pentapotassium salt] *CAS 192140-58-2* | 336 | 505 |
Citations
View all 4 citations: Citation Explorer
Nifedipine stimulates proliferation and migration of different breast cancer cells by distinct pathways
Authors: Zhao, Tao and Guo, Dongqing and Gu, Yuchun and Ling, Yang
Journal: Molecular Medicine Reports (2017): 2259--2263
Authors: Zhao, Tao and Guo, Dongqing and Gu, Yuchun and Ling, Yang
Journal: Molecular Medicine Reports (2017): 2259--2263
Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to the development of pulmonary arterial hypertension
Authors: Guo, Dongqing and Gu, Junzhong and Jiang, Hui and Ahmed, Asif and Zhang, Zhiren and Gu, Yuchun
Journal: Journal of molecular and cellular cardiology (2016): 179--187
Authors: Guo, Dongqing and Gu, Junzhong and Jiang, Hui and Ahmed, Asif and Zhang, Zhiren and Gu, Yuchun
Journal: Journal of molecular and cellular cardiology (2016): 179--187
Nifedipine promotes the proliferation and migration of breast cancer cells
Authors: Guo, Dong-Qing and Zhang, Hao and Tan, Sheng-Jiang and Gu, Yu-Chun
Journal: PloS one (2014): e113649
Authors: Guo, Dong-Qing and Zhang, Hao and Tan, Sheng-Jiang and Gu, Yu-Chun
Journal: PloS one (2014): e113649
References
View all 84 references: Citation Explorer
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Journal: Methods Mol Biol (2006): 47
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Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220
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Authors: Eerbeek O, Mik EG, Zuurbier CJ, van 't Loo M, Donkersloot C, Ince C.
Journal: J Appl Physiol (2004): 2042
Negative inotropic effects of angiotensin II, endothelin-1 and phenylephrine in indo-1 loaded adult mouse ventricular myocytes
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Authors: Sakurai K, Norota I, Tanaka H, Kubota I, Tomoike H, Endo M.
Journal: Life Sci (2002): 1173
Usefulness of the analytic method of intracellular calcium and the problems--aequorin and indo-1 signal
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