Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Microplate Readers*

Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit

Optimized for Microplate Readers

Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit is designed to measure total amounts of nucleic acids, including double-stranded DNA (dsDNA), single-stranded DNA (ssDNA) and RNA in an easy and accurate format. The kit has all the essential reagents, including Helixyte™ Green All reagent, dilution buffer, and pre-diluted DNA standards. Helixyte™ Green All reagent is a sensitive fluorescent nucleic acid probe for measuring the total amounts of nucleic acids in a sample that may contain dsDNA, ssDNA, RNA and long oligonucleotides. Helixyte™ Green All reagent indiscriminately binds to dsDNA, ssDNA and RNA. Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit is optimized for measuring the total amounts of nucleic acids with a fluorescence microplate reader.

The total nucleic acid dose response was measured with Amplite® Fluorimetric Total Nucleic Acid Quantitation Kit in a 96-well solid black plate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17630	200 Tests	Price

References

View all 50 references: Citation Explorer

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings.

Authors:

Coole, Jackson and Kortum, Alex and Tang, Yubo and Vohra, Imran and Maker, Yajur and Kundrod, Kathryn and Natoli, Mary and Richards-Kortum, Rebecca

Journal:

Journal of visualized experiments : JoVE (2021)

Quantifying viscosity and surface tension of multicomponent protein-nucleic acid condensates.

Authors:

Alshareedah, Ibraheem and Thurston, George M and Banerjee, Priya R

Journal:

Biophysical journal (2021): 1161-1169

High-resolution visualization and quantification of nucleic acid-based therapeutics in cells and tissues using Nanoscale secondary ion mass spectrometry (NanoSIMS).

Authors:

He, Cuiwen and Migawa, Michael T and Chen, Kai and Weston, Thomas A and Tanowitz, Michael and Song, Wenxin and Guagliardo, Paul and Iyer, K Swaminathan and Bennett, C Frank and Fong, Loren G and Seth, Punit P and Young, Stephen G and Jiang, Haibo

Journal:

Nucleic acids research (2021): 1-14

Nodal metastatic load in papillary thyroid carcinoma. Morphological and molecular analysis with one-step nucleic acid amplification on more than 550 lymph nodes.

Authors:

Iglesias, Carmela and González, Oscar and Temprana-Salvador, Jordi and García-Burillo, Amparo and Caubet, Enric and Ramón Y Cajal, Santiago and Zafon, Carles

Journal:

Endocrinologia, diabetes y nutricion (2021): 346-353

Role of highly sensitive nucleic acid amplification testing for plasma cytomegalovirus DNA load in diagnosis of cytomegalovirus gastrointestinal disease among kidney transplant recipients.

Authors:

Taksinwarajarn, Touchapong and Sobhonslidsuk, Abhasnee and Kantachuvesiri, Surasak and Thongprayoon, Charat and Cheungpasitporn, Wisit and Bruminhent, Jackrapong and ,

Journal:

Transplant infectious disease : an official journal of the Transplantation Society (2021): e13635

Spectral properties

Excitation (nm)	509
Emission (nm)	527

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on October 12, 2025

Component A: Helixyte™ Green All	1 vial (200 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Nucleic Acid Standard	200 µL (100 µg/mL)