Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit *Optimized for Microplate Readers*

Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit

Optimized for Microplate Readers

Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit is designed to measure total amounts of nucleic acids, including double-stranded DNA (dsDNA), single-stranded DNA (ssDNA) and RNA in an easy and accurate format. The kit has all the essential reagents, including Helixyte™ Green All reagent, dilution buffer, and pre-diluted DNA standards. Helixyte™ Green All reagent is a sensitive fluorescent nucleic acid probe for measuring the total amounts of nucleic acids in a sample that may contain dsDNA, ssDNA, RNA and long oligonucleotides. Helixyte™ Green All reagent indiscriminately binds to dsDNA, ssDNA and RNA. Helixyte™ Green Fluorimetric Total Nucleic Acid Quantitation Kit is optimized for measuring the total amounts of nucleic acids with a fluorescence microplate reader.

The total nucleic acid dose response was measured with Amplite® Fluorimetric Total Nucleic Acid Quantitation Kit in a 96-well solid black plate.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17630	200 Tests	Price

References

View all 50 references: Citation Explorer

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings.

Authors:

Coole, Jackson and Kortum, Alex and Tang, Yubo and Vohra, Imran and Maker, Yajur and Kundrod, Kathryn and Natoli, Mary and Richards-Kortum, Rebecca

Journal:

Journal of visualized experiments : JoVE (2021)

Quantifying viscosity and surface tension of multicomponent protein-nucleic acid condensates.

Authors:

Alshareedah, Ibraheem and Thurston, George M and Banerjee, Priya R

Journal:

Biophysical journal (2021): 1161-1169

High-resolution visualization and quantification of nucleic acid-based therapeutics in cells and tissues using Nanoscale secondary ion mass spectrometry (NanoSIMS).

Authors:

He, Cuiwen and Migawa, Michael T and Chen, Kai and Weston, Thomas A and Tanowitz, Michael and Song, Wenxin and Guagliardo, Paul and Iyer, K Swaminathan and Bennett, C Frank and Fong, Loren G and Seth, Punit P and Young, Stephen G and Jiang, Haibo

Journal:

Nucleic acids research (2021): 1-14

Nodal metastatic load in papillary thyroid carcinoma. Morphological and molecular analysis with one-step nucleic acid amplification on more than 550 lymph nodes.

Authors:

Iglesias, Carmela and González, Oscar and Temprana-Salvador, Jordi and García-Burillo, Amparo and Caubet, Enric and Ramón Y Cajal, Santiago and Zafon, Carles

Journal:

Endocrinologia, diabetes y nutricion (2021): 346-353

Role of highly sensitive nucleic acid amplification testing for plasma cytomegalovirus DNA load in diagnosis of cytomegalovirus gastrointestinal disease among kidney transplant recipients.

Authors:

Taksinwarajarn, Touchapong and Sobhonslidsuk, Abhasnee and Kantachuvesiri, Surasak and Thongprayoon, Charat and Cheungpasitporn, Wisit and Bruminhent, Jackrapong and ,

Journal:

Transplant infectious disease : an official journal of the Transplantation Society (2021): e13635

Spectral properties

Excitation (nm)	509
Emission (nm)	527

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

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Page updated on August 4, 2026

Component A: Helixyte™ Green All	1 vial (200 µL)
Component B: Assay Buffer	1 bottle (50 mL)
Component C: Nucleic Acid Standard	200 µL (100 µg/mL)