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Helixyte™ Total DNA Quantification Assay Kit *4-2000 ng Broad Range*
Product key features
Helixyte™ Total DNA Quantification Assay Kit provides reliable and versatile DNA measurement across a broad range for diverse applications.
- Broad detection range: Accurately measures DNA concentrations from 4–2000 ng using a fluorescence-based assay
- High DNA specificity: Selectively quantifies DNA with minimal interference from RNA, proteins, or other contaminants
- Applications: Ideal for genomic DNA quantification, PCR sample preparation, NGS workflows, and molecular diagnostics
- Comparable alternative: Performs similarly to Qubit DNA BR Assay Kits while working with standard fluorescence microplate readers instead of a dedicated Qubit fluorometer
Product description
The Helixyte™ Total DNA Quantification Assay Kit provides a convenient, fluorescence-based method for accurately measuring DNA concentrations across a wide range. The Helixyte™ Green DNA BR reagent is formulated for maximum sensitivity and selectivity toward DNA over RNA, ensuring highly reliable results for diverse DNA types, including genomic DNA, plasmid DNA, and PCR products.
This assay supports rapid processing of multiple samples in parallel, making it ideal for high-throughput applications. To perform the assay, simply mix your DNA sample with the reagent, incubate briefly, and measure fluorescence using a standard microplate reader. The formulation is optimized to tolerate common contaminants such as salts, detergents, proteins, and free nucleotides, delivering precise and reproducible measurements even in challenging sample conditions.
Example protocol
AT A GLANCE
Add test samples (50 µL)
Add Helixyte™ Green DNA BR reagent working solution (50 µL)
Incubate at room temperature for 2 minutes
Monitor fluorescence intensity at Ex/Em = 490/530 nm
Important: The following protocol serves as an example for quantifying total DNA with Helixyte™ Green DNA BR. Ensure all components are brought to room temperature before opening. Please note that no data are available for the mutagenicity or toxicity of Helixyte™ Green DNA BR stain. As this reagent binds to nucleic acids, it should be treated as a potential mutagen and handled with appropriate care. Exercise particular caution when handling the DMSO stock solution, as DMSO is known to facilitate the absorption of organic molecules into tissues.
PREPARATION OF STANDARD SOLUTIONS
https://www.aatbio.com/tools/serial-dilution/17633
PREPARATION OF WORKING SOLUTION
Add 50 μL of Helixyte™ Green DNA BR (Component A) to 2.5 mL of DNA Assay Buffer (Component B) and mix thoroughly. Protect the working solution from light by covering it with foil or storing it in the dark.
Note: We recommend preparing this solution in a plastic container rather than glass, as the dye may adsorb to glass surfaces. For optimal results, use this solution within 2 hours of preparation.
SAMPLE EXPERIMENTAL PROTOCOL
Table 1. Layout of dsDNA standards and test samples in a solid black 96-well microplate. DS=DNA Standards (DS1-DS13, 40 to 0.0098 μg/mL); BL=Blank Control; TS=Test Samples.
BL | BL | DS8 | DS8 |
DS1 | DS1 | DS9 | DS9 |
DS2 | DS2 | DS10 | DS10 |
DS3 | DS3 | DS11 | DS11 |
DS4 | DS4 | DS12 | DS12 |
DS5 | DS5 | DS13 | DS13 |
DS6 | DS6 | TS | TS |
DS7 | DS7 | TS | TS |
Table 2. Reagent composition for each well.
Well | Volume | Reagent |
DS1–DS7 | 50 µL | Serial Dilutions (40 to 0.0098 μg/mL) |
BL | 50 µL | Assay Buffer |
TS | 50 µL | Test Sample |
Prepare DNA Standards (DS), blank controls (BL), and test samples (TS) according to the layout provided in Tables 1 and 2. For a 384-well plate, use 25 μL of reagent per well instead of 50 μL.
Add 50 μL of 2X working solution to each well containing DNA Standard, blank control, and test samples to achieve a total DNA assay volume of 100 μL/well. For a 384-well plate, add 25 μL of 2X working solution to each well, resulting in a total volume of 50 μL/well.
Incubate the reaction at room temperature for 2 minutes, protected from light.
Monitor the fluorescence intensity with a fluorescence plate reader at Ex/Em = 490/530 nm (cut off at 515nm).
For data analysis, we recommend using the Online Linear Regression Calculator available here. This tool facilitates linear, logarithmic, and semi-log regression analysis to help interpret your experimental results.
Safety data sheet